PPARGC1A sirna

PPARGC1A siRNA (Human)

Cat. Num. AAA8212382

• Gene Names: PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPARGC1A; PPARGC1A siRNA (Human); LEM6; PGC1; PGC1A; PPARGC1; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha; Ligand effect modulator 6; PPARGC1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PPARGC1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 798

• Applicable Applications for PPARGC1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PPARGC1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPARGC1A sirna

siRNA to inhibit PPARGC1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7019499
• NCBI GeneID: 10891
• NCBI Accession #: NP_037393.1
• NCBI GenBank Nucleotide #: NM_013261.3
• UniProt Accession #: Q9UBK2; Q3LIG1; Q4W5M7; Q9UN32; B7Z406; G8DM16; I3RTT5; I3RTT6; I3RTT7; I3RTT8; I3RTT9
• Molecular Weight: 77,139 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• NCBI Official Synonym Full Names: peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• NCBI Official Symbol: PPARGC1A
• NCBI Official Synonym Symbols: LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• Protein Family: Peroxisome proliferator-activated receptor gamma coactivator
• UniProt Gene Name: PPARGC1A
• UniProt Synonym Gene Names: LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
• UniProt Entry Name: PRGC1_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Uniprot Description

PGC-1 alpha: a transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor genes. Interacts with, and regulates the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). Regulates many genes involved in energy metabolism. Provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. May be involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. Its transcription is repressed by ZNF746 which binds to 'insulin response sequences' in its promoter. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p15.1

Cellular Component: nucleoplasm; PML body; DNA-directed RNA polymerase II, core complex; nucleus; cytosol

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; androgen receptor binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; RNA binding; ubiquitin protein ligase binding; transcription coactivator activity; nucleotide binding; transcription factor binding

Biological Process: circadian rhythm; response to muscle activity; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; thermoregulation; positive regulation of gluconeogenesis; regulation of transcription, DNA-dependent; flavone metabolic process; protein complex assembly; negative regulation of neuron apoptosis; circadian regulation of gene expression; negative regulation of glycolysis; transcription initiation from RNA polymerase II promoter; mitochondrion organization and biogenesis; protein stabilization; RNA splicing; organelle organization and biogenesis; androgen metabolic process; cellular respiration; regulation of circadian rhythm; gluconeogenesis; response to starvation; response to leucine; digestion; positive regulation of fatty acid oxidation; androgen receptor signaling pathway; galactose metabolic process; brown fat cell differentiation; cell glucose homeostasis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; positive regulation of histone acetylation; response to cold; mRNA processing