CHFR sirna

CHFR siRNA (Mouse)

Cat. Num. AAA8212008

• Gene Names: Chfr; RNF116; C230082M18; 5730484M20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHFR; CHFR siRNA (Mouse); E3 ubiquitin-protein ligase CHFR; Checkpoint with forkhead and RING finger domains protein; CHFR sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHFR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 664

• Applicable Applications for CHFR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHFR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHFR sirna

siRNA to inhibit CHFR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 575771743
• NCBI GeneID: 231600
• NCBI Accession #: NP_001276506.1
• NCBI GenBank Nucleotide #: NM_001289577.1
• UniProt Accession #: Q810L3; Q3TFD8; Q3U233; Q3U4U9; Q3UGJ9; Q8BJZ9; Q8BWH4
• Molecular Weight: 57,925 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CHFR isoform 1
• NCBI Official Synonym Full Names: checkpoint with forkhead and ring finger domains
• NCBI Official Symbol: Chfr
• NCBI Official Synonym Symbols: RNF116; C230082M18; 5730484M20Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase CHFR
• UniProt Protein Name: E3 ubiquitin-protein ligase CHFR
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Chfr
• UniProt Entry Name: CHFR_MOUSE

Uniprot Description

CHFR: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Ubiquitous. Belongs to the CHFR family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Cellular Component: PML body; nucleus

Molecular Function: protein binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; metal ion binding; ribosomal S6-glutamic acid ligase activity; nucleotide binding; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; modification-dependent protein catabolic process; mitosis; protein polyubiquitination; protein destabilization; cell division; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; protein ubiquitination; mitotic cell cycle; cell cycle