Testing Data
(Lane 1: Detects GST-hSAP30L fusion protein in NIH3T3 cells. Lane 2: Detects wild-type transfected hSAP30L and endogenous hSAP30L in HEK293 cells. Lane 3: Detects endogenous hSAP30L in non-transfected MCF-7 cells. Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g. NIH3T3 cells), but does not detect endogenous non-human SAP30L. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kD and the endogenous hSAP30L band runs with an apparent molecular wieght of 21-22kD. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be due to cleavage product, degradation and/or product or post-translational modification.)
Rabbit anti-Human SAP30L Polyclonal Antibody | anti-SAP30L antibody
SAP30L (Sin3-associated Protein 30-like)
Gene Names
SAP30L; NS4ATP2; FLJ11526; FLJ23595; FLJ36497; DKFZp667L2214
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SAP30L; Polyclonal Antibody; SAP30L (Sin3-associated Protein 30-like); Anti -SAP30L (Sin3-associated Protein 30-like); anti-SAP30L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with residues 165-183 (VKSNKSRLDQKSEGGKQLE) of human SAP30L. Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g., NIH3T3 cells), but does not detect endogenous non-human SAP30L.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-SAP30L antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Western Blot: Use at 1:500-1:1000
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Lane 1: Detects GST-hSAP30L fusion protein in NIH3T3 cells. Lane 2: Detects wild-type transfected hSAP30L and endogenous hSAP30L in HEK293 cells. Lane 3: Detects endogenous hSAP30L in non-transfected MCF-7 cells. Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g. NIH3T3 cells), but does not detect endogenous non-human SAP30L. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kD and the endogenous hSAP30L band runs with an apparent molecular wieght of 21-22kD. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be due to cleavage product, degradation and/or product or post-translational modification.)
Testing Data
(Lane 1: Detects GST-hSAP30L fusion protein in NIH3T3 cells. Lane 2: Detects wild-type transfected hSAP30L and endogenous hSAP30L in HEK293 cells. Lane 3: Detects endogenous hSAP30L in non-transfected MCF-7 cells. Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g. NIH3T3 cells), but does not detect endogenous non-human SAP30L. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kD and the endogenous hSAP30L band runs with an apparent molecular wieght of 21-22kD. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be due to cleavage product, degradation and/or product or post-translational modification.)
Related Product Information for anti-SAP30L antibody
A novel TGF- beta up-regulated mRNA species, the Sin3-associated protein 30-like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30 it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.
The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kD and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22kD. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or product or post-translational modification.
The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kD and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22kD. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or product or post-translational modification.
Product Categories/Family for anti-SAP30L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
20,877 Da
NCBI Official Full Name
SAP30-like
NCBI Official Synonym Full Names
SAP30-like
NCBI Official Symbol
SAP30L
NCBI Official Synonym Symbols
NS4ATP2; FLJ11526; FLJ23595; FLJ36497; DKFZp667L2214
NCBI Protein Information
histone deacetylase complex subunit SAP30L; OTTHUMP00000160641; OTTHUMP00000224330; OTTHUMP00000224331; sin3-associated protein p30-like; Sin3A associated protein p30-like; sin3 corepressor complex subunit SAP30L; HCV non-structural protein 4A-transactivated protein 2
UniProt Protein Name
Histone deacetylase complex subunit SAP30L
Protein Family
UniProt Gene Name
SAP30L
UniProt Synonym Gene Names
NS4ATP2
UniProt Entry Name
SP30L_HUMAN
Uniprot Description
SAP30L: Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus. Belongs to the SAP30 family.
Protein type: Nucleolus
Chromosomal Location of Human Ortholog: 5q33.2
Cellular Component: nucleoplasm; nucleolus
Molecular Function: DNA binding; metal ion binding
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; regulation of gene expression, epigenetic
Research Articles on SAP30L
Similar Products
Product Notes
The SAP30L sap30l (Catalog #AAA617672) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAP30L (Sin3-associated Protein 30-like) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAP30L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: Use at 1:500-1:1000. Researchers should empirically determine the suitability of the SAP30L sap30l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP30L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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