Rabbit NF-kappaB p65 (pS468) Polyclonal Antibody | anti-RELA antibody

Anti-NF-kappaB p65 (pS468) Phospho Antibody

Cat. Num. AAA821173

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human, Mouse, Rat, Bovine, Monkey, Pig
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: NF-kappaB p65 (pS468); Polyclonal Antibody; Anti-NF-kappaB p65 (pS468) Phospho Antibody; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; anti-RELA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Monkey, Pig
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of NF-kappaB p65 (pS468) protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 548

• Applicable Applications for anti-RELA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NF-kappaB p65. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of NF-kappaB p65 (pS468) expression in HeLa TNFa-treated (A), NIH3T3 LPS-treated (B), rat brain (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NF-kappaB p65 (pS468) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-RELA antibody

Rabbit polyclonal antibody to NF-kappaB p65 (pS468)

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 59,910 Da
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: v-rel avian reticuloendotheliosis viral oncogene homolog A
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Transcription factor; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to cAMP; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; response to insulin stimulus; toll-like receptor 5 signaling pathway; response to organic substance; hair follicle development; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus; aging; response to drug; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; acetaldehyde metabolic process; liver development; response to amino acid stimulus; toll-like receptor 2 signaling pathway; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; positive regulation of interleukin-12 biosynthetic process; response to muramyl dipeptide; response to mechanical stimulus; regulation of inflammatory response; toll-like receptor signaling pathway; cellular defense response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; response to cobalamin; negative regulation of apoptosis