GOT1 sirna

GOT1 siRNA (Rat)

Cat. Num. AAA8211227

• Gene Names: Got1; cCAT; Aspat; Gaspat; cAspAT
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GOT1; GOT1 siRNA (Rat); Aspartate aminotransferase cytoplasmic; cAspAT; Cysteine aminotransferase cytoplasmic; Cysteine transaminase cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A; GOT1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GOT1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 413

• Applicable Applications for GOT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GOT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GOT1 sirna

siRNA to inhibit GOT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 401461792
• NCBI GeneID: 24401
• NCBI Accession #: NP_036703.2
• NCBI GenBank Nucleotide #: NM_012571.2
• UniProt Accession #: P13221; Q64570; Q6P721
• Molecular Weight: 46,429 Da
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1, soluble
• NCBI Official Symbol: Got1
• NCBI Official Synonym Symbols: cCAT; Aspat; Gaspat; cAspAT
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Protein transport protein
• UniProt Gene Name: Got1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_RAT

NCBI Description

cytosolic aspartate aminotransferase isozyme; enzyme activity in liver and kidney increase in response to glucocorticoids [RGD, Feb 2006]

Uniprot Description

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Transferase; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.1; Amino Acid Metabolism - arginine and proline; EC 2.6.1.3

Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol

Molecular Function: cysteine transaminase activity; carboxylic acid binding; phosphatidylserine decarboxylase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; aspartate biosynthetic process; 2-oxoglutarate metabolic process; dicarboxylic acid metabolic process; cellular response to insulin stimulus; glycerol biosynthetic process; fatty acid homeostasis; aspartate catabolic process; aspartate metabolic process; glutamate catabolic process to 2-oxoglutarate