APOBEC1 sirna

APOBEC1 siRNA (Human)

Cat. Num. AAA8210610

• Gene Names: APOBEC1; BEDP; HEPR; CDAR1; APOBEC-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOBEC1; APOBEC1 siRNA (Human); C->U-editing enzyme APOBEC-1; Apolipoprotein B mRNA-editing enzyme 1; HEPR; APOBEC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APOBEC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 236

• Applicable Applications for APOBEC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APOBEC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOBEC1 sirna

siRNA to inhibit APOBEC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 752507737
• NCBI GeneID: 339
• NCBI Accession #: NP_001291495.1
• NCBI GenBank Nucleotide #: NM_001304566.1
• UniProt Accession #: P41238; Q9UE64; Q9UM71
• Molecular Weight: 28,192 Da
• NCBI Official Full Name: C->U-editing enzyme APOBEC-1 isoform a
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
• NCBI Official Symbol: APOBEC1
• NCBI Official Synonym Symbols: BEDP; HEPR; CDAR1; APOBEC-1
• NCBI Protein Information: C->U-editing enzyme APOBEC-1
• UniProt Protein Name: C->U-editing enzyme APOBEC-1
• Protein Family: APOBEC1 complementation factor
• UniProt Gene Name: APOBEC1
• UniProt Entry Name: ABEC1_HUMAN

NCBI Description

This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]

Uniprot Description

APOBEC1: apolipoprotein B mRNA editing enzyme. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. Expressed exclusively in the small intestine.

Protein type: EC 3.5.4.-; RNA processing; RNA-binding; Cell cycle regulation; Hydrolase

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; zinc ion binding; RNA binding; AU-rich element binding; cytidine deaminase activity

Biological Process: response to drug; mRNA stabilization; response to osmotic stress; regulation of cell proliferation; RNA processing; response to ethanol; cellular response to insulin stimulus; cytidine to uridine editing; lipoprotein biosynthetic process; response to zinc ion; lipoprotein transport; mRNA modification; response to gamma radiation; gene expression; cytidine deamination; lipid metabolic process; response to calcium ion; mRNA processing; defense response to virus