NDEL1 sirna

NDEL1 siRNA (Human)

Cat. Num. AAA8209679

• Gene Names: NDEL1; EOPA; NDE2; NUDEL; MITAP1; NDE1L1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NDEL1; NDEL1 siRNA (Human); EOPA; MITAP1; NUDEL; Nuclear distribution protein nudE-like 1; Protein Nudel; Mitosin-associated protein 1; NDEL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NDEL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 328

• Applicable Applications for NDEL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDEL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NDEL1 sirna

siRNA to inhibit NDEL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71284108
• NCBI GeneID: 81565
• NCBI Accession #: NP_001020750.1
• NCBI GenBank Nucleotide #: NM_001025579.2
• UniProt Accession #: Q9GZM8; Q86T80; Q8TAR7; Q9UH50; B3KP93; D3DTS0; J3QT32
• Molecular Weight: 37,039 Da
• NCBI Official Full Name: nuclear distribution protein nudE-like 1 isoform A
• NCBI Official Synonym Full Names: nudE neurodevelopment protein 1-like 1
• NCBI Official Symbol: NDEL1
• NCBI Official Synonym Symbols: EOPA; NDE2; NUDEL; MITAP1; NDE1L1
• NCBI Protein Information: nuclear distribution protein nudE-like 1
• UniProt Protein Name: Nuclear distribution protein nudE-like 1
• Protein Family: Nuclear distribution protein
• UniProt Gene Name: NDEL1
• UniProt Synonym Gene Names: EOPA; MITAP1; NUDEL; Protein Nudel
• UniProt Entry Name: NDEL1_HUMAN

NCBI Description

This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Mar 2012]

Uniprot Description

NDEL1: Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Belongs to the nudE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Microtubule-binding

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: kinetochore; axon hillock; centrosome; kinesin complex; microtubule; neurofilament cytoskeleton; cytoplasm; leading edge; nucleolus; nuclear envelope; spindle; cytosol

Molecular Function: protein binding; microtubule binding; beta-tubulin binding; alpha-tubulin binding

Biological Process: positive regulation of axon regeneration; cell migration; central nervous system neuron axonogenesis; positive regulation of axon extension; establishment of chromosome localization; neuron migration; nuclear envelope disassembly; microtubule nucleation; proteolysis; inner cell mass cell proliferation; retrograde axon cargo transport; chromosome segregation; mitotic centrosome separation; centrosome localization; neurofilament cytoskeleton organization and biogenesis; establishment of mitotic spindle orientation; vesicle transport along microtubule; cerebral cortex radially oriented cell migration; mitotic cell cycle