DNMT3A sirna

DNMT3A siRNA (Mouse)

Cat. Num. AAA8209341

• Gene Names: Dnmt3a; MmuIIIA
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DNMT3A; DNMT3A siRNA (Mouse); DNA; cytosine-5)-methyltransferase 3A; Dnmt3a; DNA methyltransferase MmuIIIA; DNA MTase MmuIIIA; M.MmuIIIA; DNMT3A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DNMT3A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 908

• Applicable Applications for DNMT3A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNMT3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DNMT3A sirna

siRNA to inhibit DNMT3A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 418203928
• NCBI GeneID: 13435
• NCBI Accession #: NP_001258682.1
• NCBI GenBank Nucleotide #: NM_001271753.1
• UniProt Accession #: O88508; Q3TZK8; Q3UH24; Q8CJ60; Q922J0; Q9CSE1
• Molecular Weight: 77,824 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3A isoform 1
• NCBI Official Synonym Full Names: DNA methyltransferase 3A
• NCBI Official Symbol: Dnmt3a
• NCBI Official Synonym Symbols: MmuIIIA
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3A
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3A
• Protein Family: DNA
• UniProt Gene Name: Dnmt3a
• UniProt Synonym Gene Names: Dnmt3a; DNA MTase MmuIIIA; M.MmuIIIA
• UniProt Entry Name: DNM3A_MOUSE

NCBI Description

This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]

Uniprot Description

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase; EC 2.1.1.37; Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine

Cellular Component: XY body; nucleoplasm; heterochromatin; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: transferase activity; DNA (cytosine-5-)-methyltransferase activity; identical protein binding; methyltransferase activity; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; unmethylated CpG binding; metal ion binding; chromatin binding

Biological Process: methylation; cytosine methylation within a CG sequence; genetic imprinting; regulation of transcription, DNA-dependent; maintenance of DNA methylation; methylation-dependent chromatin silencing; transcription, DNA-dependent; DNA methylation during gametogenesis; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; S-adenosylhomocysteine metabolic process; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; DNA methylation during embryonic development; DNA methylation on cytosine