CHRNA7 sirna

CHRNA7 siRNA (Mouse)

Cat. Num. AAA8206851

• Gene Names: Chrna7; Acra7; nAchR; alpha7
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHRNA7; CHRNA7 siRNA (Mouse); ACRA7; Neuronal acetylcholine receptor subunit alpha-7; CHRNA7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHRNA7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 502

• Applicable Applications for CHRNA7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHRNA7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHRNA7 sirna

siRNA to inhibit CHRNA7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156766082
• NCBI GeneID: 11441
• NCBI Accession #: NP_031416.3
• NCBI GenBank Nucleotide #: NM_007390.3
• UniProt Accession #: P49582
• Molecular Weight: 56,632 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-7
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha polypeptide 7
• NCBI Official Symbol: Chrna7
• NCBI Official Synonym Symbols: Acra7; nAchR; alpha7
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-7
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: CHRNA7-FAM7A fusion protein
• UniProt Gene Name: Chrna7
• UniProt Synonym Gene Names: Acra7
• UniProt Entry Name: ACHA7_MOUSE

Uniprot Description

nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Channel, cation

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; dendrite; postsynaptic density; dendritic spine; integral to membrane; lipid raft; asymmetric synapse; presynaptic membrane; postsynaptic membrane; growth cone; cell soma; membrane; axon; cytoplasm; apical plasma membrane; plasma membrane; synapse; dendritic shaft; cell junction; external side of plasma membrane

Molecular Function: protein homodimerization activity; beta-amyloid binding; acetylcholine-gated cation channel activity; ion channel activity; drug binding; protein kinase binding; extracellular ligand-gated ion channel activity; ATPase binding; toxin binding; protein binding; chloride channel regulator activity; acetylcholine receptor activity; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity; adenylate cyclase binding

Biological Process: response to nicotine; T cell activation; activation of MAPK activity; generation of action potential; behavioral response to nicotine; behavioral response to ethanol; signal transduction; positive regulation of heart rate in baroreceptor response to decreased systemic arterial blood pressure; synaptic transmission, cholinergic; ovulation cycle; regulation of synaptic transmission, dopaminergic; transport; calcium ion transport; positive regulation of cell proliferation; negative regulation of interleukin-6 production; associative learning; response to food; B cell activation; endocytosis; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; regulation of norepinephrine secretion; memory; cellular calcium ion homeostasis; generation of ovulation cycle rhythm; positive regulation of angiogenesis; sperm motility; regulation of membrane potential; response to ethanol; negative regulation of inflammatory response; regulation of inflammatory response; response to hypoxia; ion transport; cognition