WNT1 sirna

WNT1 siRNA (Mouse)

Cat. Num. AAA8206700

• Gene Names: Wnt1; sw; Int-1; Wnt-1; swaying
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT1; WNT1 siRNA (Mouse); INT-1; WNT-1; Proto-oncogene Wnt-1; Proto-oncogene Int-1; WNT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WNT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 370

• Applicable Applications for WNT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WNT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT1 sirna

siRNA to inhibit WNT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 139744
• NCBI GeneID: 22408
• UniProt Accession #: P04426
• NCBI Official Full Name: Proto-oncogene Wnt-1
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 1
• NCBI Official Symbol: Wnt1
• NCBI Official Synonym Symbols: sw; Int-1; Wnt-1; swaying
• NCBI Protein Information: proto-oncogene Wnt-1
• UniProt Protein Name: Proto-oncogene Wnt-1
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: Wnt1
• UniProt Synonym Gene Names: Int-1; Wnt-1

Uniprot Description

WNT1: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Oncoprotein; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region

Molecular Function: protein domain specific binding; protein binding; frizzled binding; cytokine activity; receptor binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; multicellular organismal development; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; ubiquitin-dependent SMAD protein catabolic process; signal transduction; diencephalon development; negative regulation of BMP signaling pathway; BMP signaling pathway; cerebellum formation; positive regulation of fibroblast proliferation; cell-cell signaling; positive regulation of cell proliferation; midbrain development; midbrain-hindbrain boundary maturation during brain development; myoblast fusion; positive regulation of Notch signaling pathway; neuron fate determination; inner ear morphogenesis; organ regeneration; Wnt receptor signaling pathway; embryonic axis specification; DNA damage response, signal transduction; neuron fate commitment; myotube differentiation; negative regulation of fat cell differentiation; metencephalon development; organ morphogenesis; negative regulation of cell differentiation; central nervous system morphogenesis; ureteric bud branching; midbrain-hindbrain boundary development; forebrain anterior/posterior pattern formation; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; Spemann organizer formation