CHI3L1 sirna

CHI3L1 siRNA (Rat)

Cat. Num. AAA8205815

• Gene Names: Chi3l1; Chil1; GP-39; CGP-39
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHI3L1; CHI3L1 siRNA (Rat); Chitinase-3-like protein 1; Cartilage glycoprotein 39; CGP-39; GP-39; CHI3L1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CHI3L1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 370

• Applicable Applications for CHI3L1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CHI3L1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHI3L1 sirna

siRNA to inhibit CHI3L1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61657901
• NCBI GeneID: 89824
• NCBI Accession #: NP_446012.1
• NCBI GenBank Nucleotide #: NM_053560.1
• UniProt Accession #: Q9WTV1; Q5BJR6
• Molecular Weight: 42,392 Da
• NCBI Official Full Name: chitinase-3-like protein 1
• NCBI Official Synonym Full Names: chitinase 3-like 1 (cartilage glycoprotein-39)
• NCBI Official Symbol: Chi3l1
• NCBI Official Synonym Symbols: Chil1; GP-39; CGP-39
• NCBI Protein Information: chitinase-3-like protein 1
• UniProt Protein Name: Chitinase-3-like protein 1
• Protein Family: Chitinase-3-like protein
• UniProt Gene Name: Chi3l1
• UniProt Synonym Gene Names: CGP-39; GP-39
• UniProt Entry Name: CH3L1_RAT

NCBI Description

human homolog plays a role in vascular smooth muscle cell migration and adhesion [RGD, Feb 2006]

Uniprot Description

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung ().