SOX7 sirna

SOX7 siRNA (Human)

Cat. Num. AAA8205115

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SOX7; SOX7 siRNA (Human); Transcription factor SOX-7; SOX7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SOX7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 388

• Applicable Applications for SOX7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SOX7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SOX7 sirna

siRNA to inhibit SOX7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13899269
• NCBI GeneID: 83595
• NCBI Accession #: NP_113627.1
• NCBI GenBank Nucleotide #: NM_031439.3
• UniProt Accession #: Q9BT81; Q53YD0; B4DKV0
• Molecular Weight: 48,522 Da
• NCBI Official Full Name: transcription factor SOX-7
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 7
• NCBI Official Symbol: SOX7
• NCBI Protein Information: transcription factor SOX-7
• UniProt Protein Name: Transcription factor SOX-7
• Protein Family: Transcription factor
• UniProt Gene Name: SOX7
• UniProt Entry Name: SOX7_HUMAN

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may play a role in tumorigenesis. A similar protein in mice is involved in the regulation of the wingless-type MMTV integration site family (Wnt) pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX7: Binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. May be required for the survival of both hematopoietic and endothelial precursors during specification. Competes with GATA4 for binding and activation of the FGF3 promoter. Represses Wnt/beta-catenin-stimulated transcription, probably by targeting CTNNB1 to proteasomal degradation. Binds the DNA sequence 5'- AACAAT-3'.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; endoderm formation; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; negative regulation of transcription, DNA-dependent