CAMK2B sirna

CAMK2B siRNA (Mouse)

Cat. Num. AAA8204667

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAMK2B; CAMK2B siRNA (Mouse); CAMK2D; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CAMK2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 542

• Applicable Applications for CAMK2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CAMK2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAMK2B sirna

siRNA to inhibit CAMK2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226693349
• NCBI GeneID: 12323
• NCBI Accession #: NP_031621.3
• NCBI GenBank Nucleotide #: NM_007595.5
• UniProt Accession #: P28652; Q5SVH9
• Molecular Weight: 60,461 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit beta isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II, beta
• NCBI Official Symbol: Camk2b
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit beta
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit beta
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: Camk2b
• UniProt Synonym Gene Names: Camk2d; CaM kinase II subunit beta; CaMK-II subunit beta
• UniProt Entry Name: KCC2B_MOUSE

Uniprot Description

CAMK2B: a widely expressed protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group. Functions autonomously after Ca2+/calmodulin-binding and autophosphorylation. Involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban, an endogenous inhibitor of SERCA2. The holoenzyme is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2. Interacts with MPDZ. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle and induced during exercise. Eight isoforms of the human protein are produced by alternative splicing

Protein type: EC 2.7.11.17; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; CAMK2 family

Cellular Component: cytoskeleton; sarcoplasmic reticulum; membrane; dendrite; cytoplasm; postsynaptic density; perikaryon; spindle midzone; cytosol

Molecular Function: calmodulin binding; transferase activity; protein serine/threonine kinase activity; protein binding; phospholipase binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of long-term neuronal synaptic plasticity; nervous system development; protein amino acid autophosphorylation; cell projection morphogenesis; protein amino acid phosphorylation; regulation of synaptic transmission, cholinergic; inhibitory G-protein coupled receptor phosphorylation; peptidyl-serine phosphorylation; response to cadmium ion; calcium ion transport; cell differentiation; neuromuscular process controlling balance; phosphorylation; positive regulation of phospholipase A2 activity; G1/S transition of mitotic cell cycle