CDC40 sirna

CDC40 siRNA (Human)

Cat. Num. AAA8204169

• Gene Names: CDC40; EHB3; PRP17; PRPF17
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDC40; CDC40 siRNA (Human); EHB3; PRP17; PRPF17; Pre-mRNA-processing factor 17; Cell division cycle 40 homolog; EH-binding protein 3; Ehb3; PRP17 homolog; hPRP17; CDC40 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDC40 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 579

• Applicable Applications for CDC40 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDC40 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDC40 sirna

siRNA to inhibit CDC40 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7706657
• NCBI GeneID: 51362
• NCBI Accession #: NP_056975.1
• NCBI GenBank Nucleotide #: NM_015891.2
• UniProt Accession #: O60508; O75471; Q5SRN0; Q9UPG1; B2RBC5
• Molecular Weight: 65,521 Da
• NCBI Official Full Name: pre-mRNA-processing factor 17
• NCBI Official Synonym Full Names: cell division cycle 40
• NCBI Official Symbol: CDC40
• NCBI Official Synonym Symbols: EHB3; PRP17; PRPF17
• NCBI Protein Information: pre-mRNA-processing factor 17
• UniProt Protein Name: Pre-mRNA-processing factor 17
• UniProt Gene Name: CDC40
• UniProt Synonym Gene Names: EHB3; PRP17; PRPF17; Ehb3; hPRP17
• UniProt Entry Name: PRP17_HUMAN

NCBI Description

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC40: Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing.

Protein type: Spliceosome; RNA processing; Motility/polarity/chemotaxis; RNA splicing

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; spliceosome

Molecular Function: protein binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription