CCAR2 sirna

CCAR2 siRNA (Mouse)

Cat. Num. AAA8204104

• Gene Names: Ccar2; mKIAA1967; 2610301G19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCAR2; CCAR2 siRNA (Mouse); Cell cycle and apoptosis regulator protein 2; Cell division cycle and apoptosis regulator protein 2; CCAR2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CCAR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 922

• Applicable Applications for CCAR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CCAR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCAR2 sirna

siRNA to inhibit CCAR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226958577
• NCBI GeneID: 219158
• NCBI Accession #: NP_666167.3
• NCBI GenBank Nucleotide #: NM_146055.3
• UniProt Accession #: Q8VDP4; Q6PIB1; Q8BWR5; Q8C0F0; Q8R3G6
• Molecular Weight: 103,002 Da
• NCBI Official Full Name: cell cycle and apoptosis regulator protein 2
• NCBI Official Synonym Full Names: cell cycle activator and apoptosis regulator 2
• NCBI Official Symbol: Ccar2
• NCBI Official Synonym Symbols: mKIAA1967; 2610301G19Rik
• NCBI Protein Information: cell cycle and apoptosis regulator protein 2
• UniProt Protein Name: Cell cycle and apoptosis regulator protein 2
• Protein Family: Cell cycle and apoptosis regulator protein
• UniProt Gene Name: Ccar2
• UniProt Entry Name: CCAR2_MOUSE

Uniprot Description

DBC-1: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Component of the DBIRD complex. Interacts with ZNF326/ZIRD; the interaction is direct. Interacts (via N-terminus) with SIRT1. Interacts (via N-terminus) with SUV39H1; this interaction abolishes the interaction with SIRT1. Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Tumor suppressor; Nuclear receptor co-regulator; Apoptosis; Mitochondrial

Cellular Component: spliceosome; mitochondrial matrix; cytoplasm; nuclear chromatin; nucleus

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding

Biological Process: positive regulation of apoptosis; apoptosis; RNA splicing; rhythmic process; regulation of protein stability; regulation of circadian rhythm; cell cycle; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-dependent; negative regulation of catalytic activity; mitochondrial fragmentation during apoptosis; mRNA processing; response to DNA damage stimulus; regulation of RNA elongation; response to UV