Rabbit anti-Human, Rat WAVE2 Polyclonal Antibody | anti-WASF2 antibody

WAVE2 (Scar2)

Cat. Num. AAA610176

• Gene Names: WASF2; SCAR2; WAVE2; dJ393P12.2
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Serum; Serum
• Synonyms: WAVE2; Polyclonal Antibody; WAVE2 (Scar2); Anti -WAVE2 (Scar2); anti-WASF2 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WAVE2/Scar2, Mr ~68kD. An additional band, Mr ~64kD, is observed with longer exposures or when loading larger amounts of cell lysate. This band is not inhibited with the immunizing peptide. Species Crossreactivity: rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in 0.05% sodium azide, before the addition of glycerol to 30%.

• Applicable Applications for anti-WASF2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:2000 of this lot detected WAVE2/Scar2 in RIPA lysates from HL60 cells. HL60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with W0951 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunogen: Synthetic peptide corresponding to aa186-200 (C-NRGNVNPRKIKTRKE) of human WAVE2/Scar2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(HL60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS610176 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates WAVE2/Scar2 (68kD).)

Related Product Information for anti-WASF2 antibody

Scar/WAVE, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold.

Product Categories/Family for anti-WASF2 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 319655728
• NCBI GeneID: 10163
• NCBI Accession #: NP_001188333.1
• NCBI GenBank Nucleotide #: NM_001201404.1
• UniProt Accession #: Q05BU7; Q8NBU0; Q9Y6W5
• Molecular Weight: 21,410 Da
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 2 isoform 2
• NCBI Official Synonym Full Names: WAS protein family, member 2
• NCBI Official Symbol: WASF2
• NCBI Official Synonym Symbols: SCAR2; WAVE2; dJ393P12.2
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 2; IMD2; protein WAVE-2; OTTHUMP00000003471; WASP family protein member 2; WASP family Verprolin-homologous protein 2; verprolin homology domain-containing protein 2; suppressor of cyclic-AMP receptor (WASP-family)
• UniProt Protein Name: WASF2 protein
• Protein Family: Wiskott-Aldrich syndrome protein family
• UniProt Gene Name: WASF2
• UniProt Entry Name: Q05BU7_HUMAN

NCBI Description

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

WAVE2: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with BRK1. Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. Belongs to the SCAR/WAVE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: ruffle; lamellipodium; early endosome; intracellular; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: protein binding; protein complex binding; actin binding

Biological Process: lamellipodium biogenesis; G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of stress fiber formation; actin filament-based movement; ameboidal cell migration; Rac protein signal transduction; innate immune response; angiogenesis; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway