FIG4 sirna

FIG4 siRNA (Mouse)

Cat. Num. AAA8203420

• Gene Names: Fig4; Sac3; AI326867; A530089I17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FIG4; FIG4 siRNA (Mouse); KIAA0274; SAC3; Polyphosphoinositide phosphatase; Phosphatidylinositol 3.5-bisphosphate 5-phosphatase; SAC domain-containing protein 3; FIG4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FIG4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 907

• Applicable Applications for FIG4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommend transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FIG4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships with blue ice. Store at -20 degree C for one year.

Related Product Information for FIG4 sirna

siRNA to inhibit FIG4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19527220
• NCBI GeneID: 103199
• NCBI Accession #: NP_598760.1
• NCBI GenBank Nucleotide #: NM_133999.1
• UniProt Accession #: Q91WF7; Q6A092
• NCBI Official Full Name: polyphosphoinositide phosphatase
• NCBI Official Synonym Full Names: FIG4 homolog (S. cerevisiae)
• NCBI Official Symbol: Fig4
• NCBI Official Synonym Symbols: Sac3; AI326867; A530089I17Rik
• NCBI Protein Information: polyphosphoinositide phosphatase
• UniProt Protein Name: Polyphosphoinositide phosphatase
• Protein Family: Polyphosphoinositide phosphatase
• UniProt Gene Name: Fig4
• UniProt Synonym Gene Names: Kiaa0274; Sac3
• UniProt Entry Name: FIG4_MOUSE

Uniprot Description

SAC3: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes. Defects in FIG4 are the cause of Charcot-Marie-Tooth disease type 4J (CMT4J). CMT4J is a recessive demyelinating, severe form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. Defects in FIG4 are the cause of amyotrophic lateral sclerosis type 11 (ALS11). ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.

Protein type: EC 3.1.3.-

Cellular Component: Golgi apparatus; recycling endosome; membrane; endoplasmic reticulum; endosome membrane; endosome

Molecular Function: protein tyrosine/threonine phosphatase activity; phosphatidylinositol-3-phosphatase activity; inositol-1,3,4-trisphosphate 4-phosphatase activity; phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; hydrolase activity; inositol-1,4,5,6-tetrakisphosphate 6-phosphatase activity; inositol bisphosphate phosphatase activity; phosphoric ester hydrolase activity; inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity; JUN kinase phosphatase activity; transmembrane receptor protein phosphatase activity; NADP phosphatase activity; phosphatidylinositol-4-phosphate phosphatase activity; 5-amino-6-(5-phosphoribitylamino)uracil phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; inositol-4,5-bisphosphate 5-phosphatase activity; phosphohistidine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: myelin formation; pigmentation; dephosphorylation; negative regulation of myelination; neuron development; locomotory behavior; phosphatidylinositol metabolic process; vacuole organization and biogenesis