RNF212 sirna

RNF212 siRNA (Human)

Cat. Num. AAA8202289

• Gene Names: RNF212; ZHP3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF212; RNF212 siRNA (Human); Probable E3 SUMO-protein ligase RNF212; RING finger protein 212; RNF212 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RNF212 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 297

• Applicable Applications for RNF212 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RNF212 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF212 sirna

siRNA to inhibit RNF212 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 196259799
• NCBI GeneID: 285498
• NCBI Accession #: NP_001124506.1
• NCBI GenBank Nucleotide #: NM_001131034.3
• UniProt Accession #: Q495C1; Q495C0; Q86W82; Q8IY99; Q8N8U7; C9J8N0
• Molecular Weight: 30,357 Da
• NCBI Official Full Name: probable E3 SUMO-protein ligase RNF212 isoform a
• NCBI Official Synonym Full Names: ring finger protein 212
• NCBI Official Symbol: RNF212
• NCBI Official Synonym Symbols: ZHP3
• NCBI Protein Information: probable E3 SUMO-protein ligase RNF212
• UniProt Protein Name: Probable E3 SUMO-protein ligase RNF212
• Protein Family: Probable E3 SUMO-protein ligase
• UniProt Gene Name: RNF212
• UniProt Entry Name: RN212_HUMAN

NCBI Description

This gene encodes a RING finger protein that may function as a ubiquitin ligase. The encoded protein may be involved in meiotic recombination. This gene is located within a linkage disequilibrium block and polymorphisms in this gene may influence recombination rates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010]

Uniprot Description

RNF212: SUMO E3 ligase that acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May mediate sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement and plays an antagonist role with CCNB1IP1/HEI10 in the regulation of meiotic recombination. 6 human isoforms produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; SUMO ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: synaptonemal complex

Molecular Function: zinc ion binding; ligase activity

Biological Process: protein sumoylation; meiotic recombination; meiotic gene conversion; chiasma formation

Disease: Recombination Rate Quantitative Trait Locus 1