Typical Testing Data/Standard Curve (for reference only)
Sheep Placenta Growth Factor ELISA Kit | PLGF elisa kit
Sheep Placenta Growth Factor ELISA Kit
Gene Names
Pgf; PIGF; Plgf; AI854365
Reactivity
Sheep
Synonyms
Placenta Growth Factor; Sheep Placenta Growth Factor ELISA Kit; PLGF elisa kit
Reactivity
Sheep
Specificity
This assay has high sensitivity and excellent specificity for detection of PGF. No significant cross-reactivity or interference between PGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGF and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLGF elisa kit
Intended Uses: This PGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PGF. This ELISA kit for research use only!
Principle of the Assay: PGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PGF antibody and an PGF-HRP conjugate. The assay sample and buffer are incubated together with PGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGF concentration since PGF from samples and PGF-HRP conjugate compete for the anti-PGF antibody binding site. Since the number of sites is limited, as more sites are occupied by PGF from the sample, fewer sites are left to bind PGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGF concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PGF antibody and an PGF-HRP conjugate. The assay sample and buffer are incubated together with PGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGF concentration since PGF from samples and PGF-HRP conjugate compete for the anti-PGF antibody binding site. Since the number of sites is limited, as more sites are occupied by PGF from the sample, fewer sites are left to bind PGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGF concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,876 Da
NCBI Official Full Name
placenta growth factor isoform 1
NCBI Official Synonym Full Names
placental growth factor
NCBI Official Symbol
Pgf
NCBI Official Synonym Symbols
PIGF; Plgf; AI854365
NCBI Protein Information
placenta growth factor
UniProt Protein Name
Placenta growth factor
Protein Family
UniProt Gene Name
Pgf
UniProt Synonym Gene Names
Plgf; PlGF
UniProt Entry Name
PLGF_MOUSE
Uniprot Description
PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; membrane; extracellular region
Molecular Function: protein homodimerization activity; growth factor activity; protein heterodimerization activity
Biological Process: positive regulation of angiogenesis; ureteric bud branching; positive regulation of cell division; multicellular organismal development; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; sprouting angiogenesis; angiogenesis; cell differentiation
Research Articles on PLGF
Similar Products
Product Notes
The Sheep PLGF pgf (Catalog #AAA736570) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736570 ELISA Kit recognizes Sheep PLGF. It is sometimes possible for the material contained within the vial of "Placenta Growth Factor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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