POP4 sirna

POP4 siRNA (Human)

Cat. Num. AAA8202102

• Gene Names: POP4; RPP29
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POP4; POP4 siRNA (Human); RPP29; Ribonuclease P protein subunit p29; hPOP4; POP4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POP4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 220

• Applicable Applications for POP4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POP4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POP4 sirna

siRNA to inhibit POP4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5729986
• NCBI GeneID: 10775
• NCBI Accession #: NP_006618.1
• NCBI GenBank Nucleotide #: NM_006627.2
• UniProt Accession #: O95707; Q5XKL7; Q6FHW9; Q9UQQ3
• Molecular Weight: 25,425 Da
• NCBI Official Full Name: ribonuclease P protein subunit p29
• NCBI Official Synonym Full Names: POP4 homolog, ribonuclease P/MRP subunit
• NCBI Official Symbol: POP4
• NCBI Official Synonym Symbols: RPP29
• NCBI Protein Information: ribonuclease P protein subunit p29
• UniProt Protein Name: Ribonuclease P protein subunit p29
• Protein Family: RNases
• UniProt Gene Name: POP4
• UniProt Synonym Gene Names: RPP29; hPOP4
• UniProt Entry Name: RPP29_HUMAN

NCBI Description

This gene encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]

Uniprot Description

POP4: Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. May function with RPP38 to coordinate the nucleolar targeting and/or assembly of RNase P. Belongs to the eukaryotic/archaeal RNase P protein component 1 family.

Protein type: Nucleolus; EC 3.1.26.5; RNA processing; RNA-binding; Ribonuclease

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleolar ribonuclease P complex; ribonuclease MRP complex; ribonuclease P complex

Molecular Function: protein binding; RNA binding; ribonuclease MRP activity; ribonuclease P activity; ribonuclease P RNA binding

Biological Process: tRNA processing; rRNA processing; mRNA cleavage