TMEM30A sirna

TMEM30A siRNA (Rat)

Cat. Num. AAA8202033

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMEM30A; TMEM30A siRNA (Rat); CDC50A; Cell cycle control protein 50A; Transmembrane protein 30A; TMEM30A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TMEM30A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 328

• Applicable Applications for TMEM30A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TMEM30A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMEM30A sirna

siRNA to inhibit TMEM30A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51948472
• NCBI GeneID: 300857
• NCBI Accession #: NP_001004248.1
• NCBI GenBank Nucleotide #: NM_001004248.1
• UniProt Accession #: Q6AY41
• Molecular Weight: 37,173 Da
• NCBI Official Full Name: cell cycle control protein 50A
• NCBI Official Synonym Full Names: transmembrane protein 30A
• NCBI Official Symbol: Tmem30a
• NCBI Protein Information: cell cycle control protein 50A
• UniProt Protein Name: Cell cycle control protein 50A
• Protein Family: Cell cycle control protein
• UniProt Gene Name: Tmem30a
• UniProt Synonym Gene Names: Cdc50a
• UniProt Entry Name: CC50A_RAT

Uniprot Description

Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate. Required for the proper folding, assembly and ER to Golgi exit of the ATP8A2:TMEM30A flippase complex. ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth, and, reconstituted to liposomes, predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). The ATP8A1:TMEM30A flippase complex seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Required for the formation of the ATP8A2, ATP8B1 and ATP8B2 P-type ATPAse intermediate phosphoenzymes. Involved in uptake of platelet-activating factor (PAF). Can also mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A, ATP10B, ATP10D, ATP11A, ATP11B and ATP11C from ER to other membrane localizations ().