ENTPD5 sirna

ENTPD5 siRNA (Mouse)

Cat. Num. AAA8201920

• Gene Names: Entpd5; Pcph; Cd39l4; mNTPase; AI196558; AI987697; NTPDase5; ER-UDPase; NTPDase-5
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENTPD5; ENTPD5 siRNA (Mouse); CD39L4; Ectonucleoside triphosphate diphosphohydrolase 5; NTPDase 5; CD39 antigen-like 4; ER-UDPase; Guanosine-diphosphatase ENTPD5; GDPase ENTPD5; Nucleoside diphosphatase; Uridine-diphosphatase ENTPD5; UDPase ENTPD5; ENTPD5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ENTPD5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 427

• Applicable Applications for ENTPD5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ENTPD5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ENTPD5 sirna

siRNA to inhibit ENTPD5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71061462
• NCBI GeneID: 12499
• NCBI Accession #: NP_001021385.1
• NCBI GenBank Nucleotide #: NM_001026214.2
• UniProt Accession #: Q9WUZ9; O70214; Q544J4; Q8BR23; Q8CD29
• Molecular Weight: 47,102 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 5 isoform b
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 5
• NCBI Official Symbol: Entpd5
• NCBI Official Synonym Symbols: Pcph; Cd39l4; mNTPase; AI196558; AI987697; NTPDase5; ER-UDPase; NTPDase-5
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 5
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 5
• Protein Family: Ectonucleoside triphosphate diphosphohydrolase
• UniProt Gene Name: Entpd5
• UniProt Synonym Gene Names: Cd39l4; NTPDase 5; GDPase ENTPD5; UDPase ENTPD5
• UniProt Entry Name: ENTP5_MOUSE

Uniprot Description

ENTPD5: Uridine diphosphatase (UDPase) that promotes protein N- glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. Belongs to the GDA1/CD39 NTPase family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Oncoprotein; Hydrolase; EC 3.6.1.42; EC 3.6.1.6

Cellular Component: endoplasmic reticulum; extracellular region

Molecular Function: hydrolase activity; nucleoside-diphosphatase activity; guanosine-diphosphatase activity; uridine-diphosphatase activity

Biological Process: ATP metabolic process; cell proliferation; 'de novo' posttranslational protein folding; positive regulation of glycolysis; protein amino acid N-linked glycosylation; regulation of phosphoinositide 3-kinase cascade; cell growth