MYOD1 sirna

MYOD1 siRNA (Rat)

Cat. Num. AAA8201832

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYOD1; MYOD1 siRNA (Rat); MYOD; Myoblast determination protein 1; MYOD1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MYOD1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 318

• Applicable Applications for MYOD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MYOD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYOD1 sirna

siRNA to inhibit MYOD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28570184
• NCBI GeneID: 337868
• NCBI Accession #: NP_788268.1
• NCBI GenBank Nucleotide #: NM_176079.1
• UniProt Accession #: Q02346
• Molecular Weight: 34,359 Da
• NCBI Official Full Name: myoblast determination protein 1
• NCBI Official Synonym Full Names: myogenic differentiation 1
• NCBI Official Symbol: Myod1
• NCBI Protein Information: myoblast determination protein 1
• UniProt Protein Name: Myoblast determination protein 1
• Protein Family: Myoblast determination protein
• UniProt Gene Name: Myod1
• UniProt Synonym Gene Names: Myod
• UniProt Entry Name: MYOD1_RAT

NCBI Description

transcription factor; involved in muscle cell differentiation [RGD, Feb 2006]

Uniprot Description

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: Transcription factor; DNA-binding; RNA splicing

Cellular Component: nucleoplasm; transcription factor complex; myofibril; cytoplasm; nuclear chromatin; nucleus

Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; enzyme binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; chromatin binding; transcription factor binding; transcription factor activity; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle fiber adaptation; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; positive regulation of transcription, DNA-dependent; skeletal muscle fiber development; skeletal muscle atrophy; positive regulation of skeletal muscle fiber development; myoblast cell fate determination; myoblast fusion; skeletal muscle development; transcription, DNA-dependent; positive regulation of skeletal muscle regeneration; myotube differentiation; skeletal muscle regeneration; myotube cell development; cellular response to starvation; positive regulation of myoblast differentiation; myoblast differentiation; regulation of transcription from RNA polymerase II promoter; positive regulation of muscle cell differentiation; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of alternative nuclear mRNA splicing, via spliceosome