DRD1 sirna

DRD1 siRNA (Mouse)

Cat. Num. AAA8200875

• Gene Names: Drd1; Drd-1; Drd1a; Gpcr15; C030036C15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DRD1; DRD1 siRNA (Mouse); DRD1A; GPCR15; D(1A) dopamine receptor; Dopamine D1 receptor; DRD1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DRD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 446

• Applicable Applications for DRD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DRD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DRD1 sirna

siRNA to inhibit DRD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 620597353
• NCBI GeneID: 13488
• NCBI Accession #: NP_001278730.1
• NCBI GenBank Nucleotide #: NM_001291801.1
• UniProt Accession #: Q61616; Q8C8P8; B2RPW8
• Molecular Weight: 49,612 Da
• NCBI Official Full Name: D(1A) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: Drd1
• NCBI Official Synonym Symbols: Drd-1; Drd1a; Gpcr15; C030036C15Rik
• NCBI Protein Information: D(1A) dopamine receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: Drd1
• UniProt Synonym Gene Names: Drd1a; Gpcr15
• UniProt Entry Name: DRD1_MOUSE

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Cellular Component: endoplasmic reticulum; integral to plasma membrane; cell; dendrite; dendritic spine; integral to membrane; caveola; cytosol; membrane; cell soma; axon; plasma membrane; dendritic shaft; nerve terminal; intracellular; nucleus

Molecular Function: angiotensin receptor binding; G-protein alpha-subunit binding; drug binding; protein phosphatase binding; ATPase binding; G-protein coupled receptor activity; signal transducer activity; protein binding; dopamine receptor activity; protein heterodimerization activity; protein complex binding; dopamine binding; dopamine D1 receptor-like receptor activity; D3 dopamine receptor binding; receptor binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); prepulse inhibition; thermoregulation; G-protein signaling, adenylate cyclase activating pathway; adult walking behavior; protein import into nucleus; conditioned taste aversion; positive regulation of cAMP biosynthetic process; positive regulation of adenylate cyclase activity; phosphatidylinositol metabolic process; response to drug; phosphatidylinositol catabolic process; dopamine transport; dentate gyrus development; cerebral cortex GABAergic interneuron migration; adenylate cyclase activation; mating behavior; transmission of nerve impulse; social behavior; G-protein signaling, coupled to cyclic nucleotide second messenger; regulation of vasoconstriction; glucose import; operant conditioning; dopamine receptor signaling pathway; negative regulation of protein kinase activity; positive regulation of membrane potential; regulation of long-term neuronal synaptic plasticity; regulation of ion transport; synaptic transmission, dopaminergic; peristalsis; startle response; behavioral fear response; negative regulation of circadian sleep/wake cycle, sleep; generation of action potential; locomotory behavior; signal transduction; vasodilation; positive regulation of synaptic transmission, glutamatergic; synaptic transmission; intracellular protein transport; muscle contraction; behavioral response to cocaine; visual learning; feeding behavior; negative regulation of cell migration; associative learning; dopamine receptor, adenylate cyclase activating pathway; grooming behavior; striatum development; maternal behavior; hippocampus development; response to amphetamine; regulation of dopamine metabolic process; calcium-mediated signaling; learning; response to cocaine; astrocyte development; memory; G-protein coupled receptor protein signaling pathway; habituation; sensitization; positive regulation of release of sequestered calcium ion into cytosol; dopamine receptor, phospholipase C activating pathway; positive regulation of cell migration