Western Blot (WB)
(Western blot analysis of FKHR antibody (Center D469) in 293 cell line lysates (35ug/lane). FKHR (arrow) was detected using the purified Pab.)
Rabbit FKHR Polyclonal Antibody | anti-FOXO1 antibody
FKHR Antibody (Center D469)
Western Blot (WB)
(Western blot analysis of FKHR antibody (Center D469) in 293 cell line lysates (35ug/lane). FKHR (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of FKHR antibody (Center D469) in 293 cell line lysates (35ug/lane). FKHR (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
((LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with FKHR Antibody (Center D469), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using FKHR Antibody (Center D469)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunohistochemistry (IHC)
((LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with FKHR Antibody (Center D469), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using FKHR Antibody (Center D469)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Lau,C.J., Cancer Res. 69 (13), 5433-5440 (2009)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.
Protein type: Nuclear receptor co-regulator; Transcription factor; Oncoprotein; DNA-binding
Chromosomal Location of Human Ortholog: 13q14.1
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding
Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; cellular response to starvation; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein catabolic process; innate immune response; cell glucose homeostasis; autophagy; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis
Disease: Rhabdomyosarcoma 2
Research Articles on FOXO1
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Product Notes
The FOXO1 foxo1 (Catalog #AAA9214571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 454-482. The FKHR Antibody (Center D469) reacts with Human (Predicted Reactivity: Bovine, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's FKHR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the FOXO1 foxo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FKHR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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