MAP2 sirna

MAP2 siRNA (Rat)

Cat. Num. AAA8200263

• Gene Names: Map2; MAP2R; Mtap2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP2; MAP2 siRNA (Rat); MTAP2; Microtubule-associated protein 2; MAP-2; MAP2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MAP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 467

• Applicable Applications for MAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP2 sirna

siRNA to inhibit MAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 672065498
• NCBI GeneID: 25595
• NCBI Accession #: XP_008765430.1
• NCBI GenBank Nucleotide #: XM_008767208.1
• UniProt Accession #: P15146
• Molecular Weight: 52,658 Da
• NCBI Official Full Name: microtubule-associated protein 2 isoform X12
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: Map2
• NCBI Official Synonym Symbols: MAP2R; Mtap2
• NCBI Protein Information: microtubule-associated protein 2
• UniProt Protein Name: Microtubule-associated protein 2
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Map2
• UniProt Synonym Gene Names: Mtap2; MAP-2
• UniProt Entry Name: MTAP2_RAT

NCBI Description

regulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities [RGD, Feb 2006]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: microtubule; neuron projection; protein complex; smooth endoplasmic reticulum; rough endoplasmic reticulum; cell soma; postsynaptic density; cytoplasm; dendrite; nucleolus; intracellular; dendritic shaft

Molecular Function: calmodulin binding; protein binding; microtubule binding

Biological Process: axonogenesis; dendrite morphogenesis; dendrite development; response to progesterone stimulus; neurite development; response to estradiol stimulus; microtubule bundle formation; central nervous system neuron development