CXCL1 recombinant protein

CXCL1 Protein, Rat, Recombinant

Cat. Num. AAA8123338

• Gene Names: Cxcl1; Gro1; CINC-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CXCL1; CXCL1 Protein; Rat; Recombinant; Rat CXCL1/MGSA/NAP-3 Protein; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; alpha); CXCL1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala25-Lys96

• Species: Rat
• Predicted N Terminal: Ala 25
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the rat Cxcl1 (NP_110472.1)(Ala25-Lys96) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CXCL1 recombinant protein

Background: The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2 in chondrocytes and support the important role of AP-2 in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.

Product Categories/Family for CXCL1 recombinant protein

IL17 signaling pathway

References

Wang JG, et al. (2008) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons. Mol Pain. 4: 38.Acosta JC, et al. (2009) A role for CXCR2 in senescence, but what about in cancer Cancer Res. 69(6): 2167-70.Onan D, et al. (2009) The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. Endocrinology. 150(5): 2244-53.Ritzman AM, et al. (2010) The chemokine receptor CXCR2 ligand KC (CXCL1) mediates neutrophil recruitment and is critical for development of experimental Lyme arthritis and carditis. Infect Immun. 78(11): 4593-600.Bolitho C, et al. (2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer. 17(4): 929-40.Wenke AK, et al. (2011) The transcription factor AP-2 regulates CXCL1 during cartilage development and in osteoarthritis. Osteoarthritis Cartilage. 19(2): 206-12.

NCBI and Uniprot Product Information

• NCBI GI #: 13540652
• NCBI GeneID: 81503
• NCBI Accession #: NP_110472.1
• NCBI GenBank Nucleotide #: NM_030845.1
• UniProt Accession #: P14095
• Molecular Weight: 10,249 Da
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: Gro1; CINC-1
• NCBI Protein Information: growth-regulated alpha protein; C-X-C motif chemokine 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); cytokine-induced neutrophil chemoattractant 1; platelet-derived growth factor-inducible protein KC
• UniProt Protein Name: Growth-regulated alpha protein
• Protein Family: Growth-regulated alpha protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Cinc1; Gro; Scyb1; CINC-1
• UniProt Entry Name: GROA_RAT

NCBI Description

acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response [RGD, Feb 2006]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular space

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding

Biological Process: neutrophil chemotaxis; response to amphetamine; positive regulation of potassium ion transport; response to glucocorticoid stimulus; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; response to estradiol stimulus; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to heat; positive regulation of superoxide release; response to molecule of bacterial origin; response to gamma radiation; immune response; acute inflammatory response