CHST5 recombinant protein

CHST5 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123182

• Gene Names: Chst5; GST-4; Gn6st-3; AI173964; I-GlcNAc-6-ST
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CHST5; CHST5 Protein; Mouse; Recombinant (hFc Tag); Mouse CHST5/GlcNAc6ST-3 Protein (Fc Tag); AI173964 Protein; Gn6st-3 Protein; GST-4 Protein; I-GlcNAc-6-ST Protein; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5; CHST5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser27-Ser395

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Chst5 (NP_064334.1)(Ser27-Ser395) was expressed with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9910284
• NCBI GeneID: 56773
• NCBI Accession #: NP_064334.1
• NCBI GenBank Nucleotide #: NM_019950.2
• UniProt Accession #: Q9QUP4
• Molecular Weight: 44,537 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 5
• NCBI Official Synonym Full Names: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
• NCBI Official Symbol: Chst5
• NCBI Official Synonym Symbols: GST-4; Gn6st-3; AI173964; I-GlcNAc-6-ST
• NCBI Protein Information: carbohydrate sulfotransferase 5
• UniProt Protein Name: Carbohydrate sulfotransferase 5
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: Chst5
• UniProt Synonym Gene Names: Gst4; GST4; I-GlcNAc6ST; Intestinal GlcNAc-6-sulfotransferase; mIGn6ST; GlcNAc6ST-3; Gn6st-3

Uniprot Description

CHST6: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N- acetyllactosamine structures. Defects in CHST6 are the cause of macular corneal dystrophy (MCD). MCD is an autosomal recessive disease characterized by corneal opacities. Onset occurs in the first decade, usually between ages 5 and 9. The disorder is progressive. Minute, gray, punctate opacities develop. Corneal sensitivity is usually reduced. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. There are different types of MCD: MCD type I, in which there is a virtual absence of sulfated keratan sulfate (KS) in the serum and cornea, as determined by KS-specific antibodies; and MCD type II, in which the normal sulfated KS-antibody response is present in cornea and serum. MCD type I patients usually have a homozygous missense mutation, while MCD type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 8|8 E1

Cellular Component: Golgi apparatus; Golgi membrane; integral component of membrane; membrane

Molecular Function: N-acetylglucosamine 6-O-sulfotransferase activity; sulfotransferase activity; transferase activity

Biological Process: carbohydrate metabolic process; keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; sulfur compound metabolic process