M-CSF Active Protein | CSF1 active protein

M-CSF Protein, Mouse, Recombinant (ECD)

Cat. Num. AAA8123137

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Purity: >95% as determined by SDS-PAGE
• Synonyms: M-CSF; M-CSF Protein; Mouse; Recombinant (ECD); Mouse M-CSF/CSF-1 Protein (ECD); C87615 Protein; Csfm Protein; MCSF Protein; op Protein; colony stimulating factor 1 (macrophage); CSF1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu262

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 33
• Tag: Native
• Protein Construction: A DNA sequence encoding the mouse CSF1 (P07141-1)(Met1-Glu262) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Immobilized mouse CSF1 at 2ug/ml (100uL/well) can bind mouse CSF1R-Fch. The EC50 of mouse CSF1R-Fch is 60-220ng/mL.; 2. Measured in a cell proliferation assay using M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.8-4ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Immobilized mouse CSF1 at 2 ug/ml (100 ul/well) can bind mouse CSF1R-Fch, The EC50 of mouse CSF1R-Fch is 60-220 ng/mL.)

Related Product Information for CSF1 active protein

Background: Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

References

Pandit J. et al., 1992, Science. 258: 1358-62. Tokai M. et al., 2000, J Bacteriol. 182 (10): 2865-8. Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11. Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92. Cupp JS. et al., 2007, Am J Surg Pathol. 31 (6): 970-6.

NCBI and Uniprot Product Information

• NCBI GI #: 166064046
• NCBI GeneID: 12977
• NCBI Accession #: NP_001107001.1
• NCBI GenBank Nucleotide #: NM_001113529.1
• UniProt Accession #: P07141; Q3U395; Q8R3C8
• Molecular Weight: 29,353 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform 2
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1; osteopetrosis
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• Protein Family: Macrophage colony-stimulating factor
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_MOUSE

Uniprot Description

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; immune system process; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; positive regulation of odontogenesis of dentine-containing teeth; reproductive developmental process; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; positive regulation of Ras protein signal transduction; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell-matrix adhesion; regulation of ossification; positive regulation of cell migration