Colony Stimulating Factor 1, Macrophage (MCSF) Active Protein | MCSF active protein

Active Colony Stimulating Factor 1, Macrophage (MCSF)

Cat. Num. AAA2125331

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Applications: Cell culture, Activity Assays
• Purity: Greater than 95% by SDS-PAGE
• Synonyms: Colony Stimulating Factor 1; Macrophage (MCSF); Active Colony Stimulating Factor 1; MCSF/Colony Stimulating Factor 1; Macrophage; CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1; MCSF active protein

• Host: 293F Cell
• Purity/Purification: Greater than 95% by SDS-PAGE
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence: Lys33~Glu262

• Applicable Applications for MCSF active protein: Cell culture, Activity Assays
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Tag: N-terminal His Tag
• Biological Activity: Active
• Expression System: Eukaryotic expression
• Organism Species: Mouse
• Isoelectric Point: 5.1
• Subcellular Location: Secreted
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

Gene Sequencing

(Gene Sequencing (extract))

SDS-Page

(SDS-PAGE)

Activity

(The binding activity of M-CSF with M-CSF-R.)

Sequence

Related Product Information for MCSF active protein

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is asecreted cytokine which influences hematopoietic stem cells to differentiateinto macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoieticgrowth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affectsmacrophages and monocytes in several ways, including stimulating increasedphagocytic and chemotactic activity, and increased tumour cell cytotoxicity. Therole of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF-1R or M-CSF-R encoded by thec-fms proto-oncogene), M-CSF also modulates the proliferation of earlierhematopoietic progenitors and influence numerous physiological processesinvolved in immunology, metabolism, fertility and pregnancy. Besides, ColonyStimulating Factor Receptor, Macrophage (M-CSF-R) has been identified as aninteractor of M-CSF, thus a binding ELISA assay was conducted to detect theinteraction of recombinant mouse M-CSF and recombinant mouse M-CSF-R. Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100uL were then transferred to M-CSF-R coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRPlabelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of M-CSF and M-CSF-R was shown in Figure 1, and this effect was in adose dependent manner.

Product Categories/Family for MCSF active protein

Cytokine; Infection immunity; Active, eukaryotic recombinant

NCBI and Uniprot Product Information

• NCBI GI #: 166064046
• NCBI GeneID: 12977
• NCBI Accession #: NP_001107001.1
• NCBI GenBank Nucleotide #: NM_001113529.1
• UniProt Accession #: P07141; Q3U395; Q8R3C8
• Molecular Weight: 29,353 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform 2
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1; osteopetrosis
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_MOUSE

Uniprot Description

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; immune system process; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; positive regulation of odontogenesis of dentine-containing teeth; reproductive developmental process; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; positive regulation of Ras protein signal transduction; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell-matrix adhesion; regulation of ossification; positive regulation of cell migration