CNDP1 active protein

CNDP1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123058

• Gene Names: Cndp1; Cn1; AI746433
• Purity: >93% as determined by SDS-PAGE
• Synonyms: CNDP1; CNDP1 Protein; Mouse; Recombinant (His Tag); Mouse CNDP1 Protein (His Tag); AI746433 Protein; Cn1 Protein; carnosine dipeptidase 1 (metallopeptidase M20 family); CNDP1 active protein

• Host: HEK293 Cells
• Purity/Purification: >93% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr492

• Species: Mouse
• Activity: Measured by its ability to cleave carnosine (beta-Ala-L-His)in a two-step assay. The specific activity is >250pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CNDP1 (Q8BUG2)(Met 1-Tyr 492) was expressed, with an N-terminal signal peptide and a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CNDP1 active protein

Background: CNDP1, also known as carnosine dipeptidase 1, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), is a member of the M20 metalloprotease family. The CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region, which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy, for carnosine protection against the adverse effects of high glucose levels on renal cells. In humans, CNDP1 is secreted from the liver into the serum. In other mammals, including rodents, CNDP1 is expressed exclusively within the kidney and lacks a signal peptide. CNDP1 protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine (beta-alanyl-L-histidine), and is identified as human carnosinase expressed in the brain. CNDP1 has been associated with diabetic nephropathy in Europeans and European Americans, but not African-Americans. It was identified and confirmed as a risk factor, were cross-sectional and mostly in patients with type 2 diabetes. The polymorphisms of CNDP1 can be excluded as a risk factor for nephropathy in type 1 diabetes. In addition, CNDP1 is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.

References

Teufel M, et al. (2003) Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase. J Biol Chem 278(8):6521-31.Janssen B, et al. (2005) Carnosine as a protective factor in diabetic nephropathy: association with a leucine repeat of the carnosinase gene CNDP1. Diabetes 54(8):2320-7.Riedl E, et al. (2007) A CTG polymorphism in the CNDP1 gene determines the secretion of serum carnosinase in Cos-7 transfected cells. Diabetes 56(9):2410-3.Freedman BI, et al. (2007) A leucine repeat in the carnosinase gene CNDP1 is associated with diabetic end-stage renal disease in European Americans. Nephrol Dial Transplant 22(4):1131-5.Wanic K, et al. (2008) Exclusion of polymorphisms in carnosinase genes (CNDP1 and CNDP2) as a cause of diabetic nephropathy in type 1 diabetes: results of large case-control and follow-up studies. Diabetes 57(9):2547-51.McDonough CW, et al. (2009) The influence of carnosinase gene polymorphisms on diabetic nephropathy risk in African-Americans. Hum Genet. 126(2):265-75.

NCBI and Uniprot Product Information

• NCBI GI #: 157951637
• NCBI GeneID: 338403
• NCBI Accession #: NP_803233.2
• NCBI GenBank Nucleotide #: NM_177450.4
• UniProt Accession #: Q8BUG2; Q80XP5
• Molecular Weight: 55,090 Da
• NCBI Official Full Name: beta-Ala-His dipeptidase
• NCBI Official Synonym Full Names: carnosine dipeptidase 1 (metallopeptidase M20 family)
• NCBI Official Symbol: Cndp1
• NCBI Official Synonym Symbols: Cn1; AI746433
• NCBI Protein Information: beta-Ala-His dipeptidase
• UniProt Protein Name: Beta-Ala-His dipeptidase
• Protein Family: Beta-Ala-His dipeptidase
• UniProt Gene Name: Cndp1
• UniProt Synonym Gene Names: Cn1

Uniprot Description

CNDP1: a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region. [provided by RefSeq, Jul 2008]

Protein type: Amino Acid Metabolism - histidine; EC 3.4.13.20; Other Amino Acids Metabolism - beta-alanine; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18|18 E4

Cellular Component: cytosol

Molecular Function: dipeptidase activity; zinc ion binding

Biological Process: peptide catabolic process; proteolysis; regulation of cellular protein metabolic process