Ephrin A3 Active Protein | EFNA3 active protein

Ephrin A3 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122854

• Gene Names: Efna3; Epl3; EFL-2; Ehk1-L; LERK-3; AW494418
• Purity: >92% as determined by SDS-PAGE
• Synonyms: Ephrin A3; Ephrin A3 Protein; Mouse; Recombinant (His Tag); Mouse Ephrin-A3/EFNA3 Protein (His Tag); AW494418 Protein; EFL-2 Protein; Ehk1-L Protein; Epl3 Protein; LERK-3 Protein; ephrin-A3; EFNA3 active protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser205

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EphrinA3 at 1ug/ml (100uL/well) can bind mouse EPHA6 with a linear range of 6.25-400ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EFNA3 (NP_034238.1) without the pro peptide (Met 1-Ser 205) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse EphrinA3 at 1 ug/ml (100 ul/well) can bind mouse EPHA6 with a linear ranger of 6.25-400 ng/ml.)

Related Product Information for EFNA3 active protein

Background: Ephrin-A3 also known as EPH-related receptor tyrosine kinase ligand 3 or EFNA3, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A3 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: Ephrin-A3 ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A3 expressed on astrocytes activates EphA4 on the post-synaptic neuron and restricts the growth of dendritic spines through multiple pathways.

References

Klein R. (2009) Bidirectional modulation of synaptic functions by Eph/ephrin signaling. Nat Neurosci. 12(1): 15-20.Lai KO, et al. (2009) Synapse development and plasticity: roles of ephrin/Eph receptor signaling. Curr Opin Neurobiol. 19(3): 275-83.Prevost N, et al. (2002) Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred. Proc Natl Acad Sci U S A. 99(14): 9219-24.

NCBI and Uniprot Product Information

• NCBI GI #: 117935036
• NCBI GeneID: 13638
• NCBI Accession #: NP_034238.1
• NCBI GenBank Nucleotide #: NM_010108.1
• UniProt Accession #: O08545; O55217; Q08AV0
• Molecular Weight: 25,632 Da
• NCBI Official Full Name: ephrin-A3
• NCBI Official Synonym Full Names: ephrin A3
• NCBI Official Symbol: Efna3
• NCBI Official Synonym Symbols: Epl3; EFL-2; Ehk1-L; LERK-3; AW494418
• NCBI Protein Information: ephrin-A3; EHK1 ligand; EPH-related receptor tyrosine kinase ligand 3
• UniProt Protein Name: Ephrin-A3
• Protein Family: Ephrin
• UniProt Gene Name: Efna3
• UniProt Synonym Gene Names: Epl3; Eplg3; Lerk3; EHK1-L; LERK-3
• UniProt Entry Name: EFNA3_MOUSE