Rabbit PHGDH Polyclonal Antibody | anti-PHGDH antibody

Anti-PHGDH Antibody, Rabbit Polyclonal

Cat. Num. AAA8103703

• Gene Names: PHGDH; NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
• Reactivity: Human; Predicted to React with: Pongo abelii, Pig, Macfa (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PHGDH; Polyclonal Antibody; Anti-PHGDH Antibody; Rabbit Polyclonal; PHGDH Antibody; Rabbit PAb; Antigen Affinity Purified; phosphoglycerate dehydrogenase; Anti-3-PGDH Antibody; Anti-3PGDH Antibody; Anti-HEL-S-113 Antibody; Anti-NLS Antibody; Anti-NLS1 Antibody; Anti-PDG Antibody; Anti-PGAD Antibody; Anti-PGD Antibody; Anti-PGDH Antibody; Anti-PHGDHD Antibody; Anti-SERA Antibody; anti-PHGDH antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Pongo abelii, Pig, Macfa (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PHGDH
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PHGDH antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human PHGDH
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PHGDH, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PHGDH rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:57 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PHGDH in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of PHGDH in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PHGDH polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)

Immunoprecipitation (IP)

(PHGDH was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-PHGDH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PHGDH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa Observed band size :57 kDa)

Related Product Information for anti-PHGDH antibody

PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+ /NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

References

Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43.Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45.Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.

NCBI and Uniprot Product Information

• NCBI GI #: 21264510
• NCBI GeneID: 26227
• UniProt Accession #: O43175; Q5SZU3; Q9BQ01; B2RD08
• Molecular Weight: 533
• NCBI Official Full Name: D-3-phosphoglycerate dehydrogenase
• NCBI Official Synonym Full Names: phosphoglycerate dehydrogenase
• NCBI Official Symbol: PHGDH
• NCBI Official Synonym Symbols: NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
• NCBI Protein Information: D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113
• UniProt Protein Name: D-3-phosphoglycerate dehydrogenase
• UniProt Gene Name: PHGDH
• UniProt Synonym Gene Names: PGDH3; 3-PGDH
• UniProt Entry Name: SERA_HUMAN

NCBI Description

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]

Uniprot Description

PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Protein type: Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: cytosol

Molecular Function: electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity

Biological Process: neural tube development; glial cell development; glycine metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; spinal cord development; regulation of gene expression; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process

Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1