B7-1 Active Protein | CD80 active protein

B7-1 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122744

• Gene Names: Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• Purity: >90% as determined by SDS-PAGE
• Synonyms: B7-1; B7-1 Protein; Mouse; Recombinant (hFc Tag); Mouse CD80/B7-1 Protein (Fc Tag); B71 Protein; Cd28l Protein; Ly-53 Protein; Ly53 Protein; MIC17 Protein; TSA1 Protein; CD80 molecule; CD80 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys245

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 38
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse CD80 (Q00609-1) extracellular domain (Met 1-Lys 245) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human CTLA4 at 10ug/mL (100uL/well) can bind mouse CD80-Fc. The EC50 of mouse CD80-Fc is 28ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human CTLA4 at 10 ug/mL (100 ul/well) can bind mouse CD80-Fc, The EC50 of mouse CD80-Fc is 28 ng/mL.)

Related Product Information for CD80 active protein

Background: The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Product Categories/Family for CD80 active protein

Cancer Drug Targets

References

Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.

NCBI and Uniprot Product Information

• NCBI GI #: 112983684
• NCBI GeneID: 12519
• NCBI Accession #: NP_033985.3
• NCBI GenBank Nucleotide #: NM_009855.2
• UniProt Accession #: Q00609; Q61332
• Molecular Weight: 24,051 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 antigen
• NCBI Official Symbol: Cd80
• NCBI Official Synonym Symbols: B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: Cd80
• UniProt Synonym Gene Names: B7; B7

Uniprot Description

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16 B4|16 26.86 cM

Cellular Component: cell surface; external side of plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of alpha-beta T cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell proliferation; T cell costimulation