Western Blot (WB)
(Anti-CD80 rabbit polyclonal antibody at 1:500 dilution Lane A: RAW 264.7 Whole Cell Lysate Lane B: Mouse Spleen Whole Cell Lysate Lane C: Mouse Skeletal Muscle Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:72 kDa)
Rabbit anti-Mouse B7-1 Polyclonal Antibody | anti-CD80 antibody
Anti-B7-1 Antibody, Rabbit Polyclonal
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
B7-1; Polyclonal Antibody; Anti-B7-1 Antibody; Rabbit Polyclonal; CD80/B7-1 Antibody; Rabbit PAb; Antigen Affinity Purified; CD80 molecule; Anti-B71 Antibody; Anti-Cd28l Antibody; Anti-Ly-53 Antibody; Anti-Ly53 Antibody; Anti-MIC17 Antibody; Anti-TSA1 Antibody; anti-CD80 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse CD80/B7-1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD80 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse CD80/B7-1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD80/B7-1 (rM CD80/B7-1). CD80/B7-1 specific IgG was purified by Mouse CD80/B7-1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CD80 rabbit polyclonal antibody at 1:500 dilution Lane A: RAW 264.7 Whole Cell Lysate Lane B: Mouse Spleen Whole Cell Lysate Lane C: Mouse Skeletal Muscle Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:72 kDa)
Western Blot (WB)
(Anti-CD80 rabbit polyclonal antibody at 1:500 dilution Lane A: RAW 264.7 Whole Cell Lysate Lane B: Mouse Spleen Whole Cell Lysate Lane C: Mouse Skeletal Muscle Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:72 kDa)
Related Product Information for anti-CD80 antibody
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Product Categories/Family for anti-CD80 antibody
References
Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,051 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 antigen
NCBI Official Symbol
Cd80
NCBI Official Synonym Symbols
B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
NCBI Protein Information
T-lymphocyte activation antigen CD80
UniProt Protein Name
T-lymphocyte activation antigen CD80
Protein Family
UniProt Gene Name
Cd80
UniProt Synonym Gene Names
B7; B7
Uniprot Description
CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16 B4|16 26.86 cM
Cellular Component: cell surface; external side of plasma membrane
Molecular Function: protein binding
Biological Process: positive regulation of alpha-beta T cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell proliferation; T cell costimulation
Research Articles on CD80
Similar Products
Product Notes
The CD80 cd80 (Catalog #AAA8111588) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-B7-1 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B7-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the CD80 cd80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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