Mast Cell Protease-1/MCPT-1 Recombinant Protein | MCPT1 recombinant protein

Mast Cell Protease-1/MCPT-1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122620

• Gene Names: Mcpt1; Mcp-1; AV080368
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Mast Cell Protease-1/MCPT-1; Mast Cell Protease-1/MCPT-1 Protein; Mouse; Recombinant (His Tag); Mouse MCPT1 Protein (His Tag); AV080368 Protein; Mcp-1 Protein; carnitine palmitoyltransferase 1B (muscle); MCPT1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys246

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse MCPT1 (NP_032596.1)(Met 1-Lys 246) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MCPT1 recombinant protein

Background: Mast Cell Protease 1 (MMCP-1), also known as MCP-1, MCPT-1 and beta-chymase, is a member of the Chymase family of chymotrypsin-like serine proteases. MCPT-1 is a 26kDa beta-chymase that is a component of mast cell granules. It is a 226 amino acid (aa) protein that has a conserved pattern of six cysteines and one potential glycosylation site. The granule-derived mouse mast cell proteases-1 and -2 (mMCP-1 and -2) colocalize in similar quantities in mucosal mast cells but micrograms of mMCP-1 compared with nanograms of mMCP-2 are detected in peripheral blood during intestinal nematode infection. mMCP-1 isolated from serum is complexed with serpins and both the accumulation and the longevity of mMCP-1 in the blood is due to complex formation, protecting it from a pathway that rapidly clears mMCP-2, which is unable to form complexes with serpins. The mucosal mast cell (MMC) granule-specific beta-chymase, mouse mast cell protease-1 (mMCP-1), is released systemically into the bloodstream early in nematode infection before parasite-specific IgE responses develop and TGF-beta1 induces the constitutive release of mMCP-1 by homologs of MMC in vitro. Expression of mMCP-1 is largely restricted to intraepithelial MMC and is thought to play a role in the regulation of epithelial permeability. Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C). MCPT-1 is upregulated in the intestine in response to nematode infection, or systemic mucosa in response to anaphylaxis. Like human alpha-chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure. The intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1.

References

Pemberton AD, et al. (2003) Purification and characterization of mouse mast cell proteinase-2 and the differential expression and release of mouse mast cell proteinase-1 and -2 in vivo. Clin Exp Allergy. 33(7): 1005-12.Brown JK, et al. (2003) Constitutive secretion of the granule chymase mouse mast cell protease-1 and the chemokine, CCL2, by mucosal mast cell homologues. Clin Exp Allergy. 33(1): 132-46.Lawrence CE, et al. (2004) Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse. Gastroenterology. 127(1): 155-65.Pemberton AD, et al. (2006) Anaphylactic release of mucosal mast cell granule proteases: role of serpins in the differential clearance of mouse mast cell proteases-1 and -2. J Immunol. 176(2): 899-904.

NCBI and Uniprot Product Information

• NCBI GI #: 6678836
• NCBI GeneID: 17224
• NCBI Accession #: NP_032596.1
• NCBI GenBank Nucleotide #: NM_008570.1
• UniProt Accession #: P11034
• Molecular Weight: 27,013 Da
• NCBI Official Full Name: mast cell protease 1
• NCBI Official Synonym Full Names: mast cell protease 1
• NCBI Official Symbol: Mcpt1
• NCBI Official Synonym Symbols: Mcp-1; AV080368
• NCBI Protein Information: mast cell protease 1
• UniProt Protein Name: Mast cell protease 1
• UniProt Gene Name: Mcpt1
• UniProt Synonym Gene Names: mMCP-1

Uniprot Description

Has a chymotrypsin-like activity.