Rabbit anti-Human BOK Polyclonal Antibody | anti-BOK antibody

BOK, NT (BOK, BCL2L9, Bcl-2-related ovarian killer protein, Bcl-2-like protein 9) (APC)

Cat. Num. AAA6278726

• Gene Names: BOK; BOKL; BCL2L9
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BOK; Polyclonal Antibody; NT (BOK; BCL2L9; Bcl-2-related ovarian killer protein; Bcl-2-like protein 9) (APC); Bcl-2-like protein 9; anti-BOK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-BOK antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BOK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BOK.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(BOK Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BOK antibody detected the BOK protein (arrow).)

Related Product Information for anti-BOK antibody

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero-or homodimers and act as anti-and pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains all four BCL-2 like domains (BH1, 2, 3 and 4) and is a pro-apoptotic BCL-2 protein identified in the ovary.

Product Categories/Family for anti-BOK antibody

Antibodies; Apoptosis

References

Stein, J.L., et al. Neuroimage 53(3):1160-1174(2010). Ray, J.E., et al. Cell Death Differ. 17(5):846-859(2010). Zhang, H., et al. Int. J. Oncol. 34(4):1135-1138(2009). Rodriguez, J.M., et al. J. Biol. Chem. 281(32):22729-22735(2006). Bartholomeusz, G., et al. Mol. Carcinog. 45(2):73-83(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 14210524
• NCBI GeneID: 666
• NCBI Accession #: NP_115904.1
• NCBI GenBank Nucleotide #: NM_032515.4
• UniProt Accession #: Q9UMX3
• Molecular Weight: 23,280 Da
• NCBI Official Full Name: bcl-2-related ovarian killer protein
• NCBI Official Synonym Full Names: BCL2-related ovarian killer
• NCBI Official Symbol: BOK
• NCBI Official Synonym Symbols: BOKL; BCL2L9
• NCBI Protein Information: bcl-2-related ovarian killer protein; hBOK; bcl2-L-9; bcl-2-like protein 9
• UniProt Protein Name: Bcl-2-related ovarian killer protein
• Protein Family: Bcl-2-related ovarian killer protein
• UniProt Gene Name: BOK
• UniProt Synonym Gene Names: BCL2L9; hBOK; Bcl2-L-9
• UniProt Entry Name: BOK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family. [provided by RefSeq, Sep 2011]

Uniprot Description

BOK: May play a role in apoptosis. Belongs to the Bcl-2 family.

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; mitochondrial membrane; cytoplasm; integral to membrane; nucleus

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: caspase activation; cell proliferation; neuron apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; male gonad development; oligodendrocyte differentiation; brain development