SIGIRR recombinant protein

SIGIRR Protein, Mouse, Recombinant (His & hFc Tag)

Cat. Num. AAA8122544

• Gene Names: Sigirr; TIR8; AI256711
• Purity: >95% as determined by SDS-PAGE
• Synonyms: SIGIRR; SIGIRR Protein; Mouse; Recombinant (His & hFc Tag); Mouse SIGIRR/TIR8 Protein (His & Fc Tag); AI256711 Protein; TIR8 Protein; single immunoglobulin and toll-interleukin 1 receptor (TIR) domain; SIGIRR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro238

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the mouse SIGIRR (NP_075546.2)(Met 1-His 117) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SIGIRR recombinant protein

Background: Single Ig IL-1-related receptor (SIGIRR) or TIR8 is a member of Toll-like receptor-interleukin 1 receptor signaling (TLR-IL-1R) receptor superfamily. Although SIGIRR/TIR8 shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR/TIR8 has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR/TIR8 was mainly expressed in mouse and human epithelial tissues such as kidney, lung and gut. Resting and activated T and B lymphocytes and monocytes-macrophages expressed little or no SIGIRR/TIR8, with the exception of the mouse GG2EE macrophage line. Inflammation is enhanced in SIGIRR-deficient mice. SIGIRR negatively modulates immune responses. Inflammation is enhanced in SIGIRR-deficient mice, as shown by their enhanced chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge.

References

Wald D, et al. (2003) SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat Immunol. 4(9): 920-7.Polentarutti N, et al. (2003) Unique pattern of expression and inhibition of IL-1 signaling by the IL-1 receptor family member TIR8/SIGIRR. Eur Cytokine Netw. 14(4): 211-8.Wald D, et al. (2003) SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat Immunol. 4(9): 920-7.

NCBI and Uniprot Product Information

• NCBI GI #: 110625701
• NCBI GeneID: 24058
• NCBI Accession #: NP_075546.2
• NCBI GenBank Nucleotide #: NM_023059.3
• UniProt Accession #: Q9JLZ8; Q52L48
• Molecular Weight: 46,159 Da
• NCBI Official Full Name: single Ig IL-1-related receptor
• NCBI Official Synonym Full Names: single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
• NCBI Official Symbol: Sigirr
• NCBI Official Synonym Symbols: TIR8; AI256711
• NCBI Protein Information: single Ig IL-1-related receptor; toll/interleukin-1 receptor 8; single Ig IL-1R-related protein; single Ig IL-1R-related molecule; single Ig IL-1 receptor related protein; single immunoglobulin domain-containing IL1R-related protein
• UniProt Protein Name: Single Ig IL-1-related receptor
• Protein Family: Single Ig IL-1-related receptor
• UniProt Gene Name: Sigirr
• UniProt Synonym Gene Names: Tir8; TIR8
• UniProt Entry Name: SIGIR_MOUSE

Uniprot Description

Function: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP

By similarity. Ref.4

Subunit structure: Interacts with IL1R1, IRAK1, TLR4, TLR5, TLR9 and TRAF6. Upon IL-1 stimulation found in a complex at least composed of IL1R1, SIGIRR, MYD88, IRAK1 and TRAF6. Upon stimulation with LPC found in a complex at least composed of TLR4, SIG1IR, MYD88, IRAK1 and TRAF6. Interacts with PALM3

By similarity.

Subcellular location: Membrane; Single-pass type III membrane protein

Potential.

Tissue specificity: Expressed at high levels in kidney, and at moderate levels in colon, small intestine, lung, spleen and liver. Not expressed in brain and muscle. Expressed at high levels in epithelial cells, at moderate levels in splenocytes, and at low or undetectable levels in fibroblasts or endothelial cells. Expressed in mucosal and dendritic cells. Ref.1 Ref.4 Ref.6

Induction: Down-regulated by LPS. Down-regulated during inflammation by inhibition of an SP1-mediated pathway. Ref.4 Ref.6

Sequence similarities: Belongs to the interleukin-1 receptor family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 TIR domain.

Sequence caution: The sequence AAF26200.1 differs from that shown. Reason: Frameshift at positions 244 and 275.