SDS-PAGE
SIGIRR / TIR8 recombinant protein
Recombinant Mouse SIGIRR / TIR8 Protein (His & Fc tag)
Gene Names
Sigirr; TIR8; AI256711
Purity
> 95 % as determined by SDS-PAGE
Synonyms
SIGIRR / TIR8; Recombinant Mouse SIGIRR / TIR8 Protein (His & Fc tag); AI256711; MGC102426; TIR8; SIGIRR / TIR8 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
409
Application Notes
The recombinant mouse SIGIRR is a disulfide-linked homodimeric protein. The reduced monomer consists of 365 amino acids and has a predicted molecular mass of 40.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmSIGIRR/Fc monomer is approximately 60-65 kDa due to glycosylation.
Predicted N Terminal
Met
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for SIGIRR / TIR8 recombinant protein
Background: Single Ig IL-1-related receptor (SIGIRR) or TIR8 is a member of Toll-like receptor-interleukin 1 receptor signaling (TLR-IL-1R) receptor superfamily. Although SIGIRR/TIR8 shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR/TIR8 has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR/TIR8 was mainly expressed in mouse and human epithelial tissues such as kidney, lung and gut. Resting and activated T and B lymphocytes and monocytes-macrophages expressed little or no SIGIRR/TIR8, with the exception of the mouse GG2EE macrophage line. Inflammation is enhanced in SIGIRR-deficient mice. SIGIRR negatively modulates immune responses. Inflammation is enhanced in SIGIRR-deficient mice, as shown by their enhanced chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge.
Description: A DNA sequence encoding the mouse SIGIRR (NP_075546.2) (Met 1-His 117) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Description: A DNA sequence encoding the mouse SIGIRR (NP_075546.2) (Met 1-His 117) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,753 Da
NCBI Official Full Name
single Ig IL-1-related receptor
NCBI Official Synonym Full Names
single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
NCBI Official Symbol
Sigirr
NCBI Official Synonym Symbols
TIR8; AI256711
NCBI Protein Information
single Ig IL-1-related receptor
UniProt Protein Name
Single Ig IL-1-related receptor
UniProt Gene Name
Sigirr
UniProt Synonym Gene Names
Tir8; TIR8
Uniprot Description
SIGIRR: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 7|7 F5
Cellular Component: membrane
Biological Process: acute-phase response; negative regulation of chemokine biosynthetic process; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of transcription factor activity
Research Articles on SIGIRR / TIR8
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Product Notes
The SIGIRR / TIR8 sigirr (Catalog #AAA2547150) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse SIGIRR is a disulfide-linked homodimeric protein. The reduced monomer consists of 365 amino acids and has a predicted molecular mass of 40.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmSIGIRR/Fc monomer is approximately 60-65 kDa due to glycosylation. Researchers should empirically determine the suitability of the SIGIRR / TIR8 sigirr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGIRR / TIR8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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