B7-H4 Recombinant Protein | VTCN1 recombinant protein

B7-H4 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122465

• Gene Names: Vtcn1; B7x; B7h4; B7s1; BC032925
• Purity: >95% as determined by SDS-PAGE
• Synonyms: B7-H4; B7-H4 Protein; Mouse; Recombinant (His Tag); Mouse VTCN1/B7H4 Protein (His Tag); B7h4 Protein; B7s1 Protein; B7x Protein; BC032925 Protein; V-set domain containing T cell activation inhibitor 1; VTCN1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe29-Ser256

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Phe 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse VTCN1 (NP_848709.2)(Phe29-Ser256) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for VTCN1 recombinant protein

Background: V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type-III membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X/VTCN1/B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X/VTCN1/B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X/VTCN1/B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6/interleukin-6 and IL10/interleukin-10 and inhibited by CSF2/GM-CSF and IL4/interleukin-4 on antigen-presenting cells.

Product Categories/Family for VTCN1 recombinant protein

Cancer Drug Targets

References

Zang X, et al. (2003) B7x: a widely expressed B7 family member that inhibits T cell activation. Proc Natl Acad Sci U S A. 100(18): 10388-92.Suh WK, et al. (2006) Generation and characterization of B7-H4/B7S1/B7x-deficient mice. Mol Cell Biol. 26(17): 6403-11.Zang X, et al. (2007) B7-H3 and B7x are highly expressed in human prostate cancer and associated with disease spread and poor outcome. Proc Natl Acad Sci U S A. 104(49):19458-63.

NCBI and Uniprot Product Information

• NCBI GI #: 260166624
• NCBI GeneID: 242122
• NCBI Accession #: NP_848709.2
• NCBI GenBank Nucleotide #: NM_178594.3
• UniProt Accession #: Q7TSP5; Q7TPH5; Q8K091
• Molecular Weight: 30,875 Da
• NCBI Official Full Name: V-set domain containing T-cell activation inhibitor 1
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: Vtcn1
• NCBI Official Synonym Symbols: B7x; B7h4; B7s1; BC032925
• NCBI Protein Information: V-set domain containing T-cell activation inhibitor 1
• UniProt Protein Name: V-set domain containing T-cell activation inhibitor 1
• Protein Family: V-set domain containing T-cell activation inhibitor
• UniProt Gene Name: Vtcn1
• UniProt Synonym Gene Names: B7-H4

Uniprot Description

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3|3 F2.2

Cellular Component: external side of plasma membrane

Molecular Function: receptor binding

Biological Process: negative regulation of T cell activation; positive regulation of T cell proliferation; response to protozoan