B7-H4 / B7S1 / B7x recombinant protein

Recombinant Mouse B7-H4 / B7S1 / B7x Protein (Fc tag)

Cat. Num. AAA2547210

• Gene Names: Vtcn1; B7x; B7h4; B7s1; BC032925
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: B7-H4 / B7S1 / B7x; Recombinant Mouse B7-H4 / B7S1 / B7x Protein (Fc tag); Vtcn1; B7h4; B7s1; B7x; BC032925; B7-H4 / B7S1 / B7x recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 283

• Application Notes: The secreted recombinant mouse B7H4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 469 amino acids and has a calculated molecular mass of 52.2 kDa. The apparent molecular mass of rmB7H4/Fc monomer is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: phe 29
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for B7-H4 / B7S1 / B7x recombinant protein

Background: V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.

Description: A DNA sequence encoding the mature form of mouse B7H4 (Q7TSP5) (phe 29-Ser 256) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 260166624
• NCBI GeneID: 242122
• NCBI Accession #: NP_848709.2
• NCBI GenBank Nucleotide #: NM_178594.3
• UniProt Accession #: Q7TSP5; Q7TPH5; Q8K091
• Molecular Weight: 30,875 Da
• NCBI Official Full Name: V-set domain containing T-cell activation inhibitor 1
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: Vtcn1
• NCBI Official Synonym Symbols: B7x; B7h4; B7s1; BC032925
• NCBI Protein Information: V-set domain containing T-cell activation inhibitor 1
• UniProt Protein Name: V-set domain containing T-cell activation inhibitor 1
• UniProt Gene Name: Vtcn1
• UniProt Synonym Gene Names: B7-H4

Uniprot Description

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3|3 F2.2

Cellular Component: external side of plasma membrane

Molecular Function: receptor binding

Biological Process: negative regulation of T cell activation; positive regulation of T cell proliferation; response to protozoan