Fgf8 active protein

FGF8 Protein, Human, Recombinant

Cat. Num. AAA8122048

• Gene Names: FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FGF8; FGF8 Protein; Human; Recombinant; Human FGF8a/FGF-8a Protein; AIGF Protein; FGF-8 Protein; HBGF-8 Protein; HH6 Protein; KAL6 Protein; NEWENTRY Protein; fibroblast growth factor 8 (androgen-induced); Fgf8 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln23-Arg204

• Species: Human
• Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3ug/mL.
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the human FGF8a (NP_149355.1)(Gln23-Arg204) was expressed with an initial Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3 ug/mL.)

Related Product Information for Fgf8 active protein

Background: In mammalian embryos, transient Fgf8 expression defines the developing isthmic region, lying between the midbrain and the first rhombomere, but there has been uncertainty about the existence of a distinct isthmic segment in postnatal mammals. Retinoic acid (RA) directly represses Fgf8 through a RARE-mediated mechanism that promotes repressive chromatin, thus providing valuable insight into the mechanism of RA-FGF antagonism during progenitor cell differentiation. Fgf8 encodes a key signaling factor, and its precise regulation is essential for embryo patterning.

NCBI and Uniprot Product Information

• NCBI GI #: 15147350
• NCBI GeneID: 2253
• NCBI Accession #: NP_149355.1; NP_006110.1; NP_149354.1; NP_149353.1
• NCBI GenBank Nucleotide #: NM_033165.3
• Molecular Weight: 27,715 Da
• NCBI Official Full Name: fibroblast growth factor 8 isoform A
• NCBI Official Synonym Full Names: fibroblast growth factor 8
• NCBI Official Symbol: FGF8
• NCBI Official Synonym Symbols: HH6; AIGF; KAL6; FGF-8; HBGF-8
• NCBI Protein Information: fibroblast growth factor 8

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]