Western Blot (WB)
(Western blot analysis of POU3F2 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit POU3F2 Polyclonal Antibody | anti-POU3F2 antibody
POU3F2 Antibody
Gene Names
POU3F2; BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
POU3F2; Polyclonal Antibody; POU3F2 Antibody; Brain 2; Brain specific homeobox/POU domain protein 2; Brain-2; Brain-specific homeobox/POU domain protein 2; BRN 2; Brn-2; BRN2; N Oct 3; N OCT 3 Gene; N Oct3; Nervous system specific octamer binding transcription factor; Nervous system-specific octamer-binding transcription factor N-Oct-3; OCT 7; Oct-7; OCT7; Octamer Binding Transcription Factor 7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF 7; OTF-7; OTF7; PO3F2_HUMAN; POU class 3 homeobox 2; ; POU domain class 3 transcription factor 2; POU domain; class 3; transcription factor 2; Pou3f2; POUF 3; POUF3; Protein Brn 2; anti-POU3F2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
POU3F2 antibody detects endogenous levels of total POU3F2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
443
Applicable Applications for anti-POU3F2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Expressed specifically in the neuroectodermal cell lineage.
Predicted Cross Reactivity
Pig, Bovine
Similarity
Pig (92%), Bovine (100%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of POU3F2 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of POU3F2 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-POU3F2 antibody
Description: Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
Function: Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).
Subunit Structure: Interacts with PQBP1 (PubMed:10332029). Interaction with ISL1 (By similarity).
Similarity: Belongs to the POU transcription factor family. Class-3 subfamily.
Function: Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).
Subunit Structure: Interacts with PQBP1 (PubMed:10332029). Interaction with ISL1 (By similarity).
Similarity: Belongs to the POU transcription factor family. Class-3 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 47 kDa
Predicted: 47 kDa
Predicted: 47 kDa
NCBI Official Full Name
POU domain, class 3, transcription factor 2
NCBI Official Synonym Full Names
POU class 3 homeobox 2
NCBI Official Symbol
POU3F2
NCBI Official Synonym Symbols
BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3
NCBI Protein Information
POU domain, class 3, transcription factor 2
UniProt Protein Name
POU domain, class 3, transcription factor 2
Protein Family
UniProt Gene Name
POU3F2
UniProt Synonym Gene Names
BRN2; OCT7; OTF7; Brain-2; Brn-2; Oct-7; OTF-7
NCBI Description
This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012]
Uniprot Description
Transcription factor that plays a key role in neuronal differentiation (). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes ().
Research Articles on POU3F2
Similar Products
Product Notes
The POU3F2 pou3f2 (Catalog #AAA9604122) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU3F2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POU3F2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the POU3F2 pou3f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU3F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
