BTN3A1 recombinant protein

BTN3A1 Protein, Human, Recombinant

Cat. Num. AAA8121975

• Purity: >90% as determined by SDS-PAGE
• Synonyms: BTN3A1; BTN3A1 Protein; Human; Recombinant; Human BTN3A1/CD277 Protein; BT3.1 Protein; BTF5 Protein; BTN3.1 Protein; CD277 Protein; butyrophilin subfamily 3 member A1; BTN3A1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly254

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gln 30
• Tag: C-cleavage
• Protein Construction: A DNA sequence encoding the human BTN3A1 (NP_919423.1)(Met1-Gly254) was expressed with five amino acids (DDDDK) at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

(Human BTN3A1 / CD277 Protein)

Related Product Information for BTN3A1 recombinant protein

Background: BTN3A1 has the structure of a type I receptor of the Ig superfamily and is part of a family of seven BTN receptors encoded by genes in the MHC. BTN molecules are composed of two Ig domains (IgV, IgC2), a single transmembrane domain, and a large carboxyl-terminal domain termed B3.2 (or PRYSPRY) located in the cell cytoplasm. There are three human BTN3A loci, BTN3A1, BTN3A2, and BTN3A3, and clear orthologs of BTN3A molecules, now called CD277, are absent from the mouse genome. Despite its similarity to B7 molecules, BTN3A1 was proposed to act not as a coreceptor or costimulatory molecule, but rather to directly present pAg to the gammadelta TCR in a manner analogous to MHC-restricted peptide presentation. However, this model of BTN3A1 function has been challenged by conflicting data, which show pAg binding to a positively charged pocket in the cytosolic B3.2 domain, and that BTN3A1 does not directly engage the gammadelta TCR. This contradictory picture has emerged as a result of the complexity of the system and in particular by the use of endogenous and exogenous routes of Ag delivery in in vitro assays.

References

Rhodes DA, Chen H-C, Price AJ, et al. Activation of human gammadelta T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. Journal of immunology (Baltimore, Md: 1950). 2015;194(5):2390-2398.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human BTN3A1 consists of 231 amino acids and predicts a molecular mass of 24.8kDa.
• Protein Family: Butyrophilin subfamily