Endothelin B Receptor Recombinant Protein | EDNRB recombinant protein

Endothelin B Receptor Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121887

• Gene Names: EDNRB; ETB; ET-B; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Endothelin B Receptor; Endothelin B Receptor Protein; Human; Recombinant (His Tag); Human EDNRB/Endothelin B receptor Protein (His Tag); ABCDS Protein; ET-B Protein; ET-BR Protein; ETB Protein; ETBR Protein; ETRB Protein; HSCR Protein; HSCR2 Protein; WS4A Protein; endothelin receptor type B; EDNRB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys101

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu 27
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human EDNRB (NP_000106.1)(Met1-Lys101) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EDNRB recombinant protein

Background: The hypermethylation of EDNRB gene was remarkably related to infiltration and metastasis of gastric cancer and may attribute to the tumor progression. EDNRB is a new candidate tumor suppressor gene which is often down-regulated or even silenced by promoter hypermethylation in various human cancers. Low EDNRB expression played a role in the progression of ACC tumors. The autosomal recessive mutation in EDNRB may underlie a part of WS1 with the current test criteria, and supported that Hirschsprung's disease is a multifactorial genetic disease which requires additional factors.

NCBI and Uniprot Product Information

• NCBI GI #: 4557547
• NCBI GeneID: 1910
• NCBI Accession #: NP_000106.1
• NCBI GenBank Nucleotide #: NM_000115.3
• UniProt Accession #: P24530; O15343; Q59GB1; Q5W0G9; Q8NHM6; Q8NHM7; Q8NHM8; Q8NHM9; Q9UD23; Q9UQK3; A2A2Z8; A8K3T4; AAB24922
• Molecular Weight: 49,644 Da
• NCBI Official Full Name: endothelin B receptor isoform 1
• NCBI Official Synonym Full Names: endothelin receptor type B
• NCBI Official Symbol: EDNRB
• NCBI Official Synonym Symbols: ETB; ET-B; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2
• NCBI Protein Information: endothelin B receptor; endothelin receptor non-selective type
• UniProt Protein Name: Endothelin B receptor
• Protein Family: Endothelin B receptor
• UniProt Gene Name: EDNRB
• UniProt Synonym Gene Names: ETRB; ET-B; ET-BR
• UniProt Entry Name: EDNRB_HUMAN

NCBI Description

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ETB: a G protein-coupled receptor which activates a phosphatidylinoitol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutation in endothelin receptor type B gene. Essential component in the normal development of two neuronal crest-derived cell lineages. Two splice variant isoforms have been described.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q22

Cellular Component: nuclear membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: endothelin receptor activity; protein binding; peptide hormone binding; type 1 angiotensin receptor binding

Biological Process: epithelial fluid transport; cGMP-mediated signaling; regulation of fever; negative regulation of cellular protein metabolic process; response to pain; negative regulation of transcription from RNA polymerase II promoter; vein smooth muscle contraction; sensory perception of pain; response to organic cyclic substance; enteric nervous system development; vasodilation; regulation of epithelial cell proliferation; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; regulation of blood pressure; melanocyte differentiation; positive regulation of cell proliferation; regulation of pH; neural crest cell migration; aging; vasoconstriction; nervous system development; posterior midgut development; negative regulation of adenylate cyclase activity; macrophage chemotaxis; peripheral nervous system development; negative regulation of neuron maturation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis

Disease: Abcd Syndrome; Waardenburg Syndrome, Type 4a; Hirschsprung Disease, Susceptibility To, 2