VAMP3 recombinant protein

VAMP3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121694

• Gene Names: VAMP3; CEB
• Purity: >95% as determined by SDS-PAGE
• Synonyms: VAMP3; VAMP3 Protein; Human; Recombinant (His Tag); Human VAMP3/Cellubrevin Protein (His Tag); CEB Protein; vesicle-associated membrane protein 3; VAMP3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys77

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human VAMP3 (Q15836)(Met1-Lys77) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for VAMP3 recombinant protein

Background: VAMP3, also known as cellubrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Because of VAMP3 gene's high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, VAMP3 was shown to be the human equivalent of the rodent cellubrevin. In platelets VAMP3 resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.

References

Bernstein AM. et al., 1999, Blood. 93 (2): 571-9. Annaert WG. et al., 1997, J Cell Biol. 139 (6): 1397-410. Hager HA. et al., 2010, EMBO J. 29 (3): 532-45.

NCBI and Uniprot Product Information

• NCBI GI #: 2501082
• NCBI GeneID: 9341
• UniProt Accession #: Q15836; Q9BRV4
• Molecular Weight: 100
• NCBI Official Full Name: Vesicle-associated membrane protein 3
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 3
• NCBI Official Symbol: VAMP3
• NCBI Official Synonym Symbols: CEB
• NCBI Protein Information: vesicle-associated membrane protein 3; VAMP-3; cellubrevin; synaptobrevin-3
• UniProt Protein Name: Vesicle-associated membrane protein 3
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP3
• UniProt Synonym Gene Names: SYB3; VAMP-3; CEB
• UniProt Entry Name: VAMP3_HUMAN

NCBI Description

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

VAMP3: SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Belongs to the synaptobrevin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: neuron projection; cell surface; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to membrane; clathrin coated vesicle membrane; cytosol; secretory granule; SNARE complex; synaptic vesicle; recycling endosome; apical plasma membrane; plasma membrane; intracellular; cell junction

Molecular Function: SNAP receptor activity; SNARE binding; protein binding; syntaxin-1 binding

Biological Process: vesicle fusion; vesicle-mediated transport; exocytosis; neurotransmitter secretion; Golgi to plasma membrane protein transport; protein complex assembly; positive regulation of immunoglobulin secretion; positive regulation of receptor recycling; retrograde transport, endosome to Golgi; vesicle docking during exocytosis; calcium ion-dependent exocytosis