QPCT recombinant protein

QPCT Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121525

• Gene Names: QPCT; QC; GCT; sQC
• Purity: >95% as determined by SDS-PAGE
• Synonyms: QPCT; QPCT Protein; Human; Recombinant (His Tag); Human Glutaminyl cyclase/QPCT Protein (His Tag); GCT Protein; QC Protein; sQC Protein; glutaminyl-peptide cyclotransferase; QPCT recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala33-Leu361

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human QPCT (Q16769-1)(Ala33-Leu361) was fused with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for QPCT recombinant protein

Background: Glutaminyl cyclase, also known as QPCT, can promote the N-terminal cyclization reaction of N-terminal pyroglutamate(pGlu). The pGlu formation from its glutaminyl precursor is required in the maturation of numerous bioactive peptides, while the aberrant formation of pGlu may be related to several pathological processes, such as osteoporosis and amyloidotic diseases. Glutaminyl cyclase's structure reveals an alpha/beta scaffold akin to that of two-zinc exopeptidases but with several insertions and deletions, particularly in the active-site region. Glutaminyl cyclase's amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. It is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.

References

Busby WH, et al. (1987) An enzyme(s) that converts glutaminyl-peptides into pyroglutamyl-peptides. Presence in pituitary, brain, adrenal medulla, and lymphocytes. J Biol Chem. 262(18):8532-6.Bateman RC, et al. (2001) Evidence for essential histidines in human pituitary glutaminyl cyclase. Biochemistry. 40(37):11246-50.Schilling S, et al. (2002) Heterologous expression and characterization of human glutaminyl cyclase: evidence for a disulfide bond with importance for catalytic activity. Biochemistry. 41 (35):10849-57.

NCBI and Uniprot Product Information

• NCBI GI #: 6912618
• NCBI GeneID: 25797
• NCBI Accession #: NP_036545.1
• NCBI GenBank Nucleotide #: NM_012413.3
• UniProt Accession #: Q16769; Q16770; Q3KRG6; Q53TR4
• Molecular Weight: 35,421 Da
• NCBI Official Full Name: glutaminyl-peptide cyclotransferase
• NCBI Official Synonym Full Names: glutaminyl-peptide cyclotransferase
• NCBI Official Symbol: QPCT
• NCBI Official Synonym Symbols: QC; GCT; sQC
• NCBI Protein Information: glutaminyl-peptide cyclotransferase
• UniProt Protein Name: Glutaminyl-peptide cyclotransferase
• UniProt Gene Name: QPCT
• UniProt Synonym Gene Names: QC; sQC; EC
• UniProt Entry Name: QPCT_HUMAN

Uniprot Description

QPCT: Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides. Belongs to the glutaminyl-peptide cyclotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.2.5; Secreted; Secreted, signal peptide; Transferase

Chromosomal Location of Human Ortholog: 2p22.2

Molecular Function: glutaminyl-peptide cyclotransferase activity; zinc ion binding

Biological Process: peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase; protein modification process