Rabbit anti-Human QPCT Polyclonal Antibody | anti-QPCT antibody

Anti-QPCT Antibody, Rabbit Polyclonal

Cat. Num. AAA8107068

• Gene Names: QPCT; QC; GCT; sQC
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: QPCT; Polyclonal Antibody; Anti-QPCT Antibody; Rabbit Polyclonal; Glutaminyl cyclase/QPCT Antibody; Rabbit PAb; Antigen Affinity Purified; glutaminyl-peptide cyclotransferase; Anti-GCT Antibody; Anti-QC Antibody; Anti-sQC Antibody; anti-QPCT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Glutaminyl cyclase/QPCT
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-QPCT antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:4000
• Immunogen: Recombinant Human Glutaminyl cyclase/QPCT Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Glutaminyl cyclase/QPCT (rh Glutaminyl cyclase/QPCT). Glutaminyl cyclase/QPCT specific IgG was purified by Human Glutaminyl cyclase/QPCT affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human QPCT in human brain with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human QPCT in human thyroid with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-QPCT antibody

Glutaminyl cyclase, also known as QPCT, can promote the N-terminal cyclization reaction of N-terminal pyroglutamate(pGlu). The pGlu formation from its glutaminyl precursor is required in the maturation of numerous bioactive peptides, while the aberrant formation of pGlu may be related to several pathological processes, such as osteoporosis and amyloidotic diseases. Glutaminyl cyclase's structure reveals an alpha/beta scaffold akin to that of two-zinc exopeptidases but with several insertions and deletions, particularly in the active-site region. Glutaminyl cyclase's amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. It is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.

References

Busby WH, et al. (1987) An enzyme(s) that converts glutaminyl-peptides into pyroglutamyl-peptides. Presence in pituitary, brain, adrenal medulla, and lymphocytes. J Biol Chem. 262(18):8532-6.Bateman RC, et al. (2001) Evidence for essential histidines in human pituitary glutaminyl cyclase. Biochemistry. 40(37):11246-50.Schilling S, et al. (2002) Heterologous expression and characterization of human glutaminyl cyclase: evidence for a disulfide bond with importance for catalytic activity. Biochemistry. 41 (35):10849-57.

NCBI and Uniprot Product Information

• NCBI GI #: 6912618
• NCBI GeneID: 25797
• NCBI Accession #: NP_036545.1
• NCBI GenBank Nucleotide #: NM_012413.3
• UniProt Accession #: Q16769; Q16770; Q3KRG6; Q53TR4
• Molecular Weight: 35,421 Da
• NCBI Official Full Name: glutaminyl-peptide cyclotransferase
• NCBI Official Synonym Full Names: glutaminyl-peptide cyclotransferase
• NCBI Official Symbol: QPCT
• NCBI Official Synonym Symbols: QC; GCT; sQC
• NCBI Protein Information: glutaminyl-peptide cyclotransferase
• UniProt Protein Name: Glutaminyl-peptide cyclotransferase
• UniProt Gene Name: QPCT
• UniProt Synonym Gene Names: QC; sQC; EC
• UniProt Entry Name: QPCT_HUMAN

Uniprot Description

QPCT: Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides. Belongs to the glutaminyl-peptide cyclotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.2.5; Secreted; Secreted, signal peptide; Transferase

Chromosomal Location of Human Ortholog: 2p22.2

Molecular Function: glutaminyl-peptide cyclotransferase activity; zinc ion binding

Biological Process: peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase; protein modification process