Secretogranin II Recombinant Protein | SCG2 recombinant protein

Secretogranin II Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121411

• Purity: >95% as determined by SDS-PAGE
• Synonyms: Secretogranin II; Secretogranin II Protein; Human; Recombinant (His Tag); Human SCG2/Secretogranin II Protein (His Tag); CHGC Protein; EM66 Protein; SgII Protein; SN Protein; secretogranin II; SCG2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met617

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Pro 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human SCG2 (AAH22509.1)(Met1-Met617) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SCG2 recombinant protein

Background: Kit ligand, also known as Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, Kitlg and KITL, is a single-pass type I membrane protein that belongs to the SCF family. KITL/kit ligand also belongs to the family of dimeric transmembrane growth factors. The soluble form of KIT ligand is a secreted protein. Mast cells are thought to participate in a variety of immune responses, such as parasite resistance and the allergic reaction. Mast cell development depends on stem cell factor (Kit ligand) and its receptor, c-Kit. KITL/kit ligand stimulates the proliferation of mast cells. KITL/kit ligand is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Efficient cell surface presentation of KITL/kit ligand is essential for the migration, proliferation, and survival of melanocytes, germ cells, hemopoietic stem cells, and mastocytes. KITL/kit ligand acts synergistically with other cytokines, probably interleukins. KITL/kit ligand plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. It exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes. KITL/kit ligand misexpression in some hyperpigmented lesions may open the avenue for Kitl-dependent treatment of pathological skin conditions.

References

Nishida,K.et al., 2002, Blood. 99 (5):1866-9. Paulhe,F. et al., 2004,J Biol Chem. 279 (53):55545-55. Fernandez,S.M. et al., 2008,Biol Reprod. 79 (2):318-27. Wehrle-Haller,B. et al., 2003,Pigment Cell Res.16 (3):287-96.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human SCG2 comprises 612 amino acids and has a predicted molecular mass of 70.5kDa.
• Protein Family: Secretogranin