TMEM27 Recombinant Protein | CLTRN recombinant protein

TMEM27 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121370

• Gene Names: AMIGO2; ALI1; DEGA; AMIGO-2
• Purity: >85% as determined by SDS-PAGE
• Synonyms: TMEM27; TMEM27 Protein; Human; Recombinant (His Tag); Human TMEM27 Protein (His Tag); NX-17 Protein; NX17 Protein; transmembrane protein 27; CLTRN recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro141

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 15
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TMEM27 (NP_862830.1)(Met1-Pro141) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CLTRN recombinant protein

Background: TMEM27 is a membrane protein. It has been proposed as a beta cell mass biomarker since it is cleaved and shed by pancreatic beta cells. Overexpression of TMEM27 leads to increased thymidine incorporation, whereas silencing of Tmem27 using RNAi results in a reduction of cell replication. Furthermore, transgenic mice with increased expression of Tmem27 in pancreatic beta cells exhibit increased beta cell mass. TMEM27 is also important for trafficking amino acid transporters to the apical brush border of proximal tubules.

References

Pasquali L, et al. (2009) Collectrin gene screening in Turner syndrome patients with kidney malformation. J Genet. 88(1):105-8.Tosetto E, et al. (2009) Locus heterogeneity of Dent's disease: OCRL1 and TMEM27 genes in patients with no CLCN5 mutations. Pediatr Nephrol. 24(10):1967-73.Singer D, et al. (2011) Collectrin and ACE2 in renal and intestinal amino acid transport. Channels (Austin). 5(5):410-23.

NCBI and Uniprot Product Information

• NCBI GI #: 32469517
• NCBI GeneID: 347902
• NCBI Accession #: NP_862830.1
• NCBI GenBank Nucleotide #: NM_181847.4
• UniProt Accession #: Q86SJ2; Q4VBP6; Q7Z4A0; Q96CN8
• Molecular Weight: 57,934 Da
• NCBI Official Full Name: amphoterin-induced protein 2
• NCBI Official Synonym Full Names: adhesion molecule with Ig-like domain 2
• NCBI Official Symbol: AMIGO2
• NCBI Official Synonym Symbols: ALI1; DEGA; AMIGO-2
• NCBI Protein Information: amphoterin-induced protein 2
• UniProt Protein Name: Amphoterin-induced protein 2
• UniProt Gene Name: AMIGO2
• UniProt Synonym Gene Names: DEGA
• UniProt Entry Name: AMGO2_HUMAN

Uniprot Description

AMIGO2: Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas. Belongs to the immunoglobulin superfamily. AMIGO family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13.11

Molecular Function: protein binding

Biological Process: heterophilic cell adhesion; homophilic cell adhesion; negative regulation of programmed cell death